GSBS Student Publications
Title
A selected ribozyme catalyzing diverse dipeptide synthesis
UMMS Affiliation
Graduate School of Biomedical Sciences; Program in Molecular Medicine
Date
5-29-2002
Document Type
Article
Medical Subject Headings
Adenosine Monophosphate; Base Sequence; Biotin; Catalysis; Chromatography, High Pressure Liquid; Dipeptides; Kinetics; Mass Spectrometry; Molecular Sequence Data; Nuclear Magnetic Resonance, Biomolecular; Nucleic Acid Conformation; Peptide Synthases; Peptidyl Transferases; RNA, Catalytic; RNA, Ribosomal; Structure-Activity Relationship; Substrate Specificity
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The sequence of events by which protein, RNA, and DNA emerged during early biological evolution is one of the most profound questions regarding the origin of life. The contemporary role of aminoacyl-adenylates as intermediates in both ribosomal and nonribosomal peptide synthesis suggests that they may have served as substrates for uncoded peptide synthesis during early evolution. We report a highly active peptidyl transferase ribozyme family, isolated by in vitro selection, that efficiently catalyzes dipeptide synthesis by using an aminoacyl-adenylate substrate. It was characterized by sequence and structural analysis and kinetic studies. Remarkably, the ribozyme catalyzed the formation of 30 different dipeptides, the majority of rates being within 5-fold that of the Met-Phe dipeptide required by the selection. The isolation of this synthetic ribozyme fosters speculation that ribozyme-mediated uncoded peptide synthesis may have preceded coded peptide synthesis.
Rights and Permissions
Citation: Chem Biol. 2002 May;9(5):619-28.
Related Resources
Journal Title
Chemistry and biology
PubMed ID
12031668
Repository Citation
Sun, Lele; Cui, Zhiyong; Gottlieb, Robert L.; and Zhang, Biliang, "A selected ribozyme catalyzing diverse dipeptide synthesis" (2002). GSBS Student Publications. 1214.
https://escholarship.umassmed.edu/gsbs_sp/1214