Centrosomes and the Scrambled protein coordinate microtubule-independent actin reorganization
Graduate School of Biomedical Sciences; Program in Molecular Medicine and the Department of Molecular Genetics and Microbiology
Medical Subject Headings
Actins; Animals; Blastoderm; Cell Cycle Proteins; Centrosome; Colchicine; Cytochalasin D; Cytoskeleton; Drosophila; *Drosophila Proteins; Embryo, Nonmammalian; Insect Proteins; Interphase; Microtubules; Mitosis; Mutation; Polymers
Life Sciences | Medicine and Health Sciences
In Drosophila syncytial blastoderm embryos, centrosomes specify the position of actin-based interphase caps and mitotic furrows. Mutations in the scrambled locus prevent assembly of mitotic furrows, but do not block actin cap formation. The scrambled gene encodes a protein that localizes to the mitotic furrows and centrosomes. Sced localization, actin reorganization from caps into mitotic furrows, and centrosome-coordinated assembly of actin caps are not blocked by microtubule disruption. Our results indicate that centrosomes may coordinate assembly of cortical actin caps through a microtubule-independent mechanism, and that Scrambled mediates a second microtubule-independent process that drives mitotic furrow assembly.
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Citation: Nat Cell Biol. 2001 Jan;3(1):68-75. Link to article on publisher's site
DOI of Published Version
Nature cell biology
Stevenson, Victoria A.; Kramer, Joseph; Kuhn, Jesse; and Theurkauf, William E., "Centrosomes and the Scrambled protein coordinate microtubule-independent actin reorganization" (2001). GSBS Student Publications. 1202.