GSBS Student Publications
Title
Functional delineation of three groups of the ATP-dependent family of chromatin remodeling enzymes
GSBS Program
Biochemistry & Molecular Pharmacology
Publication Date
2000-04-26
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Cell Biology; Program in Molecular Medicine
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
ATP-dependent chromatin remodeling enzymes antagonize the inhibitory effects of chromatin. We compare six different remodeling complexes: ySWI/SNF, yRSC, hSWI/SNF, xMi-2, dCHRAC, and dNURF. We find that each complex uses similar amounts of ATP to remodel nucleosomal arrays at nearly identical rates. We also perform assays with arrays reconstituted with hyperacetylated or trypsinized histones and isolated histone (H3/H4)(2) tetramers. The results define three groups of the ATP-dependent family of remodeling enzymes. In addition we investigate the ability of an acidic activator to recruit remodeling complexes to nucleosomal arrays. We propose that ATP-dependent chromatin remodeling enzymes share a common reaction mechanism and that a key distinction between complexes is in their mode of regulation or recruitment.
DOI of Published Version
10.1074/jbc.M002810200
Source
J Biol Chem. 2000 Jun 23;275(25):18864-70. Link to article on publisher's site
Journal/Book/Conference Title
The Journal of biological chemistry
Related Resources
PubMed ID
10779516
Repository Citation
Boyer, Laurie A.; Logie, Colin; Bonte, Edgar; Becker, Peter B.; Wade, Paul A.; Wolffe, Alan P.; Wu, Carl; Imbalzano, Anthony N.; and Peterson, Craig L., "Functional delineation of three groups of the ATP-dependent family of chromatin remodeling enzymes" (2000). GSBS Student Publications. 119.
https://escholarship.umassmed.edu/gsbs_sp/119