Title
Sensitivity of bacteriophage RB69 DNA polymerase to N2-(p-n-butylphenyl)-2'-deoxyguanosine nucleotides
Academic Program
Biochemistry & Molecular Pharmacology
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Pharmacology and Molecular Toxicology
Publication Date
2000-01-05
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The response of bacteriophage RB69 DNA polymerase to N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate (BuPdGTP), related nucleotides and non-nucleoside inhibitors was measured and compared to values obtained for the closely related DNA polymerase from bacteriophage T4. Both enzymes showed similar responses to inhibitors in terms of Ki values and the ability to utilize BuPdGTP as a substrate. These results provide the relevance of using the recent crystal structure of RB69 DNA polymerase for analysis of BuPdGTP/B family DNA polymerase interactions.
DOI of Published Version
10.1080/07328319908044875
Source
Nucleosides Nucleotides. 1999 Oct;18(10):2193-9.
Journal/Book/Conference Title
Nucleosides and nucleotides
Related Resources
PubMed ID
10616725
Repository Citation
Stattel JM, Wright GE. (2000). Sensitivity of bacteriophage RB69 DNA polymerase to N2-(p-n-butylphenyl)-2'-deoxyguanosine nucleotides. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1080/07328319908044875. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1161