GSBS Student Publications
Title
Real-time visualization of processive myosin 5a-mediated vesicle movement in living astrocytes
Publication Date
2001-07-27
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Cellular and Molecular Physiology; Department of Physiology; Program in Molecular Medicine
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Recycling endosomes in astrocytes show hormone-regulated, actin fiber-dependent delivery to the endosomal sorting pool. Recycling vesicle trafficking was followed in real time using a fusion protein composed of green fluorescent protein coupled to the 29-kDa subunit of the short-lived, membrane-bound enzyme type 2 deiodinase. Primary endosomes budded from the plasma membrane and oscillated near the cell periphery for 1-4 min. The addition of thyroid hormone triggered the processive, centripetal movement of the recycling vesicle in linear bursts at velocities of up to 200 nm/s. Vesicle migration was hormone-specific and blocked by inhibitors of actin polymerization and myosin ATPase. Domain mapping confirmed that the hormone-dependent vesicle-binding domain was located at the C terminus of the motor. In addition, the interruption of normal dimerization of native myosin 5a monomers inactivated vesicle transport, indicating that single-headed myosin 5a motors do not transport cargo in situ. This is the first demonstration of processive hormone-dependent myosin 5a movement in living cells.
DOI of Published Version
10.1074/jbc.M103331200
Source
J Biol Chem. 2001 Sep 21;276(38):35652-9. Epub 2001 Aug 9. Link to article on publisher's site
Journal/Book/Conference Title
The Journal of biological chemistry
Related Resources
PubMed ID
11470781
Repository Citation
Stachelek, Stanley J.; Tuft, Richard A.; Lifschitz, Lawrence M.; Leonard, Deborah; Farwell, Alan P.; and Leonard, Jack L., "Real-time visualization of processive myosin 5a-mediated vesicle movement in living astrocytes" (2001). GSBS Student Publications. 1159.
https://escholarship.umassmed.edu/gsbs_sp/1159