Differences in synthesis of membrane proteins by leukemic cells from spleen and peripheral blood indicate distinct subsets of malignant cells in a patient with prolymphocytic leukemia
Graduate School of Biomedical Sciences; Department of Pharmacology; Department of Pathology; Department of Pediatrics
Medical Subject Headings
Aged; Blood Cells; Cell Transformation, Neoplastic; Cells, Cultured; Electrophoresis, Polyacrylamide Gel; Humans; Immunoglobulin M; Leukemia, Lymphoid; Lymph Nodes; Male; Membrane Proteins; Molecular Weight; Spleen
Life Sciences | Medicine and Health Sciences
Morphological and biochemical differences were demonstrated between prolymphocytic leukemia cells obtained from the spleen and peripheral blood of one patient. Peripheral blood prolymphocytes had consistently smaller nuclear-cytoplasmic ratios than did splenic prolymphocytes. Percoll gradient-purified prolymphocytes from the spleen synthesized abundant amounts of some membrane proteins which were hardly expressed by peripheral blood prolymphocytes. Peripheral blood prolymphocytes did not change their expression of membrane proteins during three days in culture. These findings are consistent with the view that prolymphocytic leukemia cells from the spleen exist, on the average, at an earlier stage of differentiation than do circulating leukemic cells, and that peripheral blood leukemic cells are frozen at a specific phase of differentiation.
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Citation: Am J Hematol. 1981 Nov;11(3):261-70.
American journal of hematology
Spiro, Robert Christopher; Ansell, Jack E.; Katayama, Isao; Muralidharan, Kamala; Sullivan, John L.; and Humphreys, Robert E., "Differences in synthesis of membrane proteins by leukemic cells from spleen and peripheral blood indicate distinct subsets of malignant cells in a patient with prolymphocytic leukemia" (1981). GSBS Student Publications. 1147.