Title
Coupling tandem affinity purification and quantitative tyrosine iodination to determine subunit stoichiometry of protein complexes
Academic Program
Biochemistry & Molecular Pharmacology
UMMS Affiliation
Graduate School of Biomedical Sciences; Program in Molecular Medicine; Program in Gene Function and Expression
Publication Date
2003-08-02
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Rapid protein purification methodologies, such as strategies involving the tandem affinity purification module, have resulted in the identification of a tremendous number of multisubunit protein complexes. Furthermore, in this modern genomic age, mass spectrometry methods are often coupled with affinity purification to identify the genes that encode each protein subunit. However, simple methodologies to determine the stoichiometry of individual subunits within a multisubunit complex have not received much attention. In this article we describe a procedure to rapidly and efficiently determine the stoichiometry of subunits within multisubunit complexes using a combination of tandem affinity purification and quantitative 125I labeling of subunit tyrosines.
DOI of Published Version
10.1016/S1046-2023(03)00094-X
Source
Methods. 2003 Sep;31(1):104-9.
Journal/Book/Conference Title
Methods (San Diego, Calif.)
Related Resources
PubMed ID
12893180
Repository Citation
Smith CL, Peterson CL. (2003). Coupling tandem affinity purification and quantitative tyrosine iodination to determine subunit stoichiometry of protein complexes. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1016/S1046-2023(03)00094-X. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1129