Title
Measuring the rates of transcriptional elongation in the female Drosophila melanogaster germ line by nuclear run-on
Academic Program
Biochemistry & Molecular Pharmacology
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Publication Date
2007-03-27
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
We adapted the nuclear run-on method to measure changes in the rate of RNA polymerase II (pol II) transcription of repetitive elements and transposons in the female germ line of Drosophila melanogaster. Our data indicate that as little as an approximately 1.5-fold change in the rate of transcription can be detected by this method. Our nuclear run-on protocol likely measures changes in transcriptional elongation, because rates of transcription decline with time, consistent with a low rate of pol II re-initiation in the isolated nuclei. Surprisingly, we find that the retrotransposon gypsy and the repetitive sequence mst40 are silenced posttranscriptionally in fly ovaries.
DOI of Published Version
10.1101/sqb.2006.71.031
Source
Cold Spring Harb Symp Quant Biol. 2006;71:335-41. Link to article on publisher's site
Journal/Book/Conference Title
Cold Spring Harbor symposia on quantitative biology
Related Resources
PubMed ID
17381314
Repository Citation
Sigova AA, Vagin VV, Zamore PD. (2007). Measuring the rates of transcriptional elongation in the female Drosophila melanogaster germ line by nuclear run-on. GSBS Student Publications. https://doi.org/10.1101/sqb.2006.71.031. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1119