Title
Probing SWI/SNF remodeling of the nucleosome by unzipping single DNA molecules
UMMS Affiliation
Graduate School of Biomedical Sciences; PetersonCornell University; Department of Molecular Medicine
Publication Date
2006-05-30
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Chromatin-remodeling enzymes can overcome strong histone-DNA interactions within the nucleosome to regulate access of DNA-binding factors to the genetic code. By unzipping individual DNA duplexes, each containing a uniquely positioned nucleosome flanked by long segments of DNA, we directly probed histone-DNA interactions. The resulting disruption-force signatures were characteristic of the types and locations of interactions and allowed measurement of the positions of nucleosomes with 2.6-base-pair (bp) precision. Nucleosomes remodeled by yeast SWI/SNF were moved bidirectionally along the DNA, resulting in a continuous position distribution. The characteristic distance of motion was approximately 28 bp per remodeling event, and each event occurred with a catalytic efficiency of 0.4 min(-1) per nM SWI/SNF. Remodeled nucleosomes had essentially identical disruption signatures to those of unremodeled nucleosomes, indicating that their overall structure remained canonical. These results impose substantial constraints on the mechanism of SWI/SNF remodeling.
DOI of Published Version
10.1038/nsmb1102
Source
Nat Struct Mol Biol. 2006 Jun;13(6):549-54. Epub 2006 May 28. Link to article on publisher's site
Journal/Book/Conference Title
Nature structural and molecular biology
Related Resources
PubMed ID
16732285
Repository Citation
Shundrovsky A, Smith CL, Lis JT, Peterson CL, Wang MD. (2006). Probing SWI/SNF remodeling of the nucleosome by unzipping single DNA molecules. GSBS Student Publications. https://doi.org/10.1038/nsmb1102. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1115