The Conserved Kinases CDK-1, GSK-3, KIN-19, and MBK-2 Promote OMA-1 Destruction to Regulate the Oocyte-to-Embryo Transition in C. elegans
Graduate School of Biomedical Sciences; Program in Molecular Medicine
Medical Subject Headings
Alleles; Amino Acid Sequence; Animals; CDC2 Protein Kinase; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Carrier Proteins; Cell Differentiation; Conserved Sequence; Embryo, Nonmammalian; Glycogen Synthase Kinase 3; Models, Biological; Molecular Sequence Data; Mutation; Nuclear Proteins; Oocytes; Phosphorylation; Protein Kinases; Protein-Tyrosine Kinases; Sequence Alignment; Signal Transduction; Wnt Proteins
Life Sciences | Medicine and Health Sciences
BACKGROUND: At the onset of embryogenesis, key developmental regulators called determinants are activated asymmetrically to specify the body axes and tissue layers. In C. elegans, this process is regulated in part by a conserved family of CCCH-type zinc finger proteins that specify the fates of early embryonic cells. The asymmetric localization of these and other determinants is regulated in early embryos through motor-dependent physical translocation as well as selective proteolysis.
RESULTS: We show here that the CCCH-type zinc finger protein OMA-1 serves as a nexus for signals that regulate the transition from oogenesis to embryogenesis. While OMA-1 promotes oocyte maturation during meiosis, destruction of OMA-1 is needed during the first cell division for the initiation of ZIF-1-dependent proteolysis of cell-fate determinants. Mutations in four conserved protein kinase genes-mbk-2/Dyrk, kin-19/CK1alpha, gsk-3, and cdk-1/CDC2-cause stabilization of OMA-1 protein, and their phenotypes are partially suppressed by an oma-1 loss-of-function mutation. OMA-1 proteolysis also depends on Cyclin B3 and on a ZIF-1-independent CUL-2-based E3 ubiquitin ligase complex, as well as the CUL-2-interacting protein ZYG-11 and the Skp1-related proteins SKR-1 and SKR-2.
CONCLUSIONS: Our findings suggest that a CDK1/Cyclin B3-dependent activity links OMA-1 proteolysis to completion of the first cell cycle and support a model in which OMA-1 functions to prevent the premature activation of cell-fate determinants until after they are asymmetrically partitioned during the first mitosis.
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Citation: Curr Biol. 2006 Jan 10;16(1):47-55. Epub 2005 Dec 15. Link to article on publisher's site
DOI of Published Version
Current biology : CB
Shirayama, Masaki; Soto, Martha C.; Ishidate, Takao; Kim, Soyoung; Nakamura, Kuniaki; Bei, Yanxia; van den Heuvel, Sander; and Mello, Craig C., "The Conserved Kinases CDK-1, GSK-3, KIN-19, and MBK-2 Promote OMA-1 Destruction to Regulate the Oocyte-to-Embryo Transition in C. elegans" (2005). GSBS Student Publications. 1110.