Cell cycle regulation of the c-Myc transcriptional activation domain
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Biology; Program in Molecular Medicine
Medical Subject Headings
Animals; Base Sequence; Binding Sites; CHO Cells; Cell Cycle; Cell Line; Cricetinae; DNA; Gene Expression Regulation; *Genes, myc; Kinetics; Molecular Sequence Data; Phosphorylation; Serine; Trans-Activation (Genetics); *Transcription, Genetic
Life Sciences | Medicine and Health Sciences
The product of the c-myc gene (c-Myc) is a sequence-specific DNA-binding protein that has previously been demonstrated to be required for cell cycle progression. Here we report that the c-Myc DNA binding site confers cell cycle regulation to a reporter gene in Chinese hamster ovary cells. The observed transactivation was biphasic with a small increase in G1 and a marked increase during the S-to-G2/M transition of the cell cycle. This cell cycle regulation of transactivation potential is accounted for, in part, by regulatory phosphorylation of the c-Myc transactivation domain. Together, these data demonstrate that c-Myc may have an important role in the progression of cells through both the G1 and G2 phases of the cell cycle.
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Citation: Mol Cell Biol. 1993 Jul;13(7):4125-36.
Molecular and cellular biology
Seth, Alpna; Gupta, Shashi; and Davis, Roger J., "Cell cycle regulation of the c-Myc transcriptional activation domain" (1993). GSBS Student Publications. 1099.