Progesterone and insulin stimulation of CPEB-dependent polyadenylation is regulated by Aurora A and glycogen synthase kinase-3
Graduate School of Biomedical Sciences; Program in Molecular Medicine
Medical Subject Headings
Amino Acid Sequence; Animals; Cell Cycle Proteins; Female; Glycogen Synthase Kinase 3; Gonadotropins, Equine; Insulin; Molecular Sequence Data; Mutagenesis, Site-Directed; Oocytes; Peptide Fragments; Phosphorylation; Poly A; Progesterone; Protein Kinases; Protein-Serine-Threonine Kinases; Transcription Factors; Xenopus Proteins; Xenopus laevis; mRNA Cleavage and Polyadenylation Factors
Life Sciences | Medicine and Health Sciences
Progesterone stimulation of Xenopus oocyte maturation requires the cytoplasmic polyadenylation-induced translation of mos and cyclin B mRNAs. One cis element that drives polyadenylation is the CPE, which is bound by the protein CPEB. Polyadenylation is stimulated by Aurora A (Eg2)-catalyzed CPEB serine 174 phosphorylation, which occurs soon after oocytes are exposed to progesterone. Here, we show that insulin also stimulates Aurora A-catalyzed CPEB S174 phosphorylation, cytoplasmic polyadenylation, translation, and oocyte maturation. However, these insulin-induced events are uniquely controlled by PI3 kinase and PKC-zeta, which act upstream of Aurora A. The intersection of the progesterone and insulin signaling pathways occurs at glycogen synthase kinase 3 (GSK-3), which regulates the activity of Aurora A. GSK-3 and Aurora A interact in vivo, and overexpressed GSK-3 inhibits Aurora A-catalyzed CPEB phosphorylation. In vitro, GSK-3 phosphorylates Aurora A on S290/291, the result of which is an autophosphorylation of serine 349. GSK-3 phosphorylated Aurora A, or Aurora A proteins with S290/291D or S349D mutations, have reduced or no capacity to phosphorylate CPEB. Conversely, Aurora A proteins with S290/291A or S349A mutations are constitutively active. These results suggest that the progesterone and insulin stimulate maturation by inhibiting GSK-3, which allows Aurora A activation and CPEB-mediated translation.
Rights and Permissions
Citation: Genes Dev. 2004 Jan 1;18(1):48-61. Link to article on publisher's site
DOI of Published Version
Genes and development
Sarkissian, Madathia; Mendez, Raul; and Richter, Joel D., "Progesterone and insulin stimulation of CPEB-dependent polyadenylation is regulated by Aurora A and glycogen synthase kinase-3" (2004). GSBS Student Publications. 1074.