GSBS Student Publications

Title

DNA immunization for influenza virus: studies using hemagglutinin- and nucleoprotein-expressing DNAs

Publication Date

1997-08-01

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Pathology

Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

DNA-based immunizations have been used to analyze the ability of DNA-expressed hemagglutinin (HA) and nucleoprotein (NP) to protect BALB/c mice against a homologous influenza virus, A/PR/8/34 (H1N1), challenge. The HA DNA, but not the NP DNA, protected mice against the lethal viral challenge. For the HA DNA, single gene gun inoculations of 0.04 microg and boosted inoculations of 0.004 microg of DNA raised complete protection. For the NP DNA, boosted gene gun immunizations of 0.4 microg of DNA and boosted intradermal or intramuscular injections of 50 microg of DNA failed to protect. The protection elicited by the HA DNA vaccine correlated with the titers of neutralizing antibody.

Source

J Infect Dis. 1997 Aug;176 Suppl 1:S50-5.

Journal/Book/Conference Title

The Journal of infectious diseases

Related Resources

Link to Article in PubMed

PubMed ID

9240695

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