Title
DNA immunization for influenza virus: studies using hemagglutinin- and nucleoprotein-expressing DNAs
Academic Program
Biochemistry & Molecular Pharmacology
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Pathology
Publication Date
1997-08-01
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
DNA-based immunizations have been used to analyze the ability of DNA-expressed hemagglutinin (HA) and nucleoprotein (NP) to protect BALB/c mice against a homologous influenza virus, A/PR/8/34 (H1N1), challenge. The HA DNA, but not the NP DNA, protected mice against the lethal viral challenge. For the HA DNA, single gene gun inoculations of 0.04 microg and boosted inoculations of 0.004 microg of DNA raised complete protection. For the NP DNA, boosted gene gun immunizations of 0.4 microg of DNA and boosted intradermal or intramuscular injections of 50 microg of DNA failed to protect. The protection elicited by the HA DNA vaccine correlated with the titers of neutralizing antibody.
DOI of Published Version
10.1086/514176
Source
J Infect Dis. 1997 Aug;176 Suppl 1:S50-5.
Journal/Book/Conference Title
The Journal of infectious diseases
Related Resources
PubMed ID
9240695
Repository Citation
Robinson HL, Boyle CM, Feltquate DM, Morin MJ, Santoro JC, Webster RG. (1997). DNA immunization for influenza virus: studies using hemagglutinin- and nucleoprotein-expressing DNAs. GSBS Student Publications. https://doi.org/10.1086/514176. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1047