Title
Critical functional role of the COOH-terminal ends of longitudinal hydrophobic strips in alpha-helices of T4 lysozyme
Academic Program
Not applicable
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Molecular Genetics; Department of Molecular Genetics and Microbiology
Publication Date
1992-09-05
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The sensitivity of bacteriophage T4 lysozyme function to amino acid substitutions at defined positions in and around the longitudinal, hydrophobic strips of 9 alpha-helices was assessed after systematic replacement of each residue in the protein with a series of 13 amino acids. The hydrophobic strips were defined by identifying the longitudinal sectors in the helices with the highest mean residue hydrophobicities. Sensitivity to mutation (the percentage of replacements leading to loss of function) was calculated for each residue in the following positions: whole protein, helices, hydrophobic strips, other positions within the helices, and various positions within the hydrophobic strips as well as their extensions beyond the helices. Substitutions at positions in the hydrophobic strips led more frequently to loss of function than substitutions in the protein as a whole. One subset, the COOH-terminal hydrophobic strip residues, is apparently critical; substitutions of these residues (but not of their NH2-terminal counterparts) led at least as frequently to loss of function as substitutions of solvent-inaccessible residues, and nearly as frequently as substitutions of the most highly conserved residues.
Source
J Biol Chem. 1992 Sep 5;267(25):17748-52.
Journal/Book/Conference Title
The Journal of biological chemistry
Related Resources
PubMed ID
1517218
Repository Citation
Rennell D, Poteete AR, Beaulieu M, Kuo DZ, Lew RA, Humphreys RE. (1992). Critical functional role of the COOH-terminal ends of longitudinal hydrophobic strips in alpha-helices of T4 lysozyme. Morningside Graduate School of Biomedical Sciences Student Publications. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1036