Phage P22 lysis genes: nucleotide sequences and functional relationships with T4 and lambda genes
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology
Medical Subject Headings
Amino Acid Sequence; Bacteriophage lambda; Base Sequence; Coliphages; DNA Restriction Enzymes; Escherichia coli; *Genes, Viral; Genetic Complementation Test; Plasmids; Protein Biosynthesis; Salmonella Phages; Species Specificity; Structure-Activity Relationship; T-Phages; Viral Proteins
Life Sciences | Medicine and Health Sciences
Wild-type and amber mutant alleles of the lysis genes of P22 were cloned and sequenced. Gene 13 encodes an 11,520-Da basic hydrophobic protein that has 89% amino acid homology to lambda S protein. Gene 19 encodes a protein that has a small degree of amino acid homology with T4 lysozyme, but we could detect no homology to lambda R or RZ proteins. The protein product of gene 19 was purified; its amino terminal amino acid sequence is as predicted by the DNA sequence. It starts with a single amino terminal methionine residue and is a basic protein with a molecular weight of 15,968. Plasmids expressing P22 gene 19, lambda genes R and RZ, and T4 gene e were constructed. All of these plasmids were able to complement both lambda R- and P22 19-.
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Citation: Virology. 1985 May;143(1):280-9.
Rennell, Dale and Poteete, Anthony R., "Phage P22 lysis genes: nucleotide sequences and functional relationships with T4 and lambda genes" (1985). GSBS Student Publications. 1034.