GSBS Student Publications
Title
Subnuclear distribution of the vitamin D receptor
GSBS Program
Biochemistry & Molecular Pharmacology
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Cell Biology
Date
4-1-1994
Document Type
Article
Medical Subject Headings
Animals; Base Sequence; Cell Nucleus; DNA; DNA-Binding Proteins; Molecular Sequence Data; Nuclear Matrix; Nuclear Proteins; Osteosarcoma; Proto-Oncogene Proteins c-jun; Rats; Receptors, Calcitriol; Transcription Factors; Tumor Cells, Cultured; Vitamin D
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The subnuclear distribution of the vitamin D receptor was investigated to begin addressing the contribution of nuclear architecture to vitamin D-responsive control of gene expression in ROS 17/2.8 rat osteosarcoma cells. The nuclear matrix is an anastomosing network of filaments that is functionally associated with DNA replication, transcription, and RNA processing. The representation of vitamin D receptor in the nuclear matrix and nonmatrix nuclear fractions was determined by the combined application of 1) sequence-specific interactions with the vitamin D receptor binding element of the rat bone-specific osteocalcin gene promoter and 2) Western blot analysis. Both methods confirmed the presence of vitamin D receptor in the nonmatrix nuclear fraction and the absence of detectable vitamin D receptors associated with the nuclear matrix. In contrast, these same nuclear matrix proteins preparations exhibited association with the general transcription factor AP-1 and a bone tissue-specific promoter binding factor NMP2. NMP-2 exhibits recognition for a promoter domain contiguous to the vitamin D-responsive element of the osteocalcin gene, although the vitamin D receptor does not appear to be a component of the nuclear matrix proteins. Interrelationships between nuclear matrix proteins and nonmatrix nuclear proteins, in mediating steroid hormone responsiveness of a vitamin D-regulated promoter, are therefore suggested.
Rights and Permissions
Citation: J Cell Biochem. 1994 Apr;54(4):494-500. Link to article on publisher's site
DOI of Published Version
10.1002/jcb.240540417
Related Resources
Journal Title
Journal of cellular biochemistry
PubMed ID
8014199
Repository Citation
Bidwell, Joseph P.; Van Wijnen, Andre J.; Fey, Edward G.; Merriman, Harold L.; Penman, Sheldon; Stein, Janet L.; Stein, Gary S.; and Lian, Jane B., "Subnuclear distribution of the vitamin D receptor" (1994). GSBS Student Publications. 100.
https://escholarship.umassmed.edu/gsbs_sp/100