Biochemistry and Molecular Pharmacology
RNA Therapeutics Institute
First Thesis Advisor
Phillip D. Zamore
piRNA, PIWI, Argonaute, Zinc-Finger, GTSF1, Spermatogenesis, RNAi, miRNAs, siRNAs, Asterix, Evolution, small RNA methylation, non-coding RNAs, small-silencing RNAs
Argonaute proteins are small DNA/RNA-guided endonucleases found in all domains of life. In animals, small RNAs of length 21–35 nucleotides direct the PIWI-clade of Argonautes to silence complementary target RNAs; these are called PIWI-interacting RNAs (piRNAs). During spermatogenesis in mice, piRNA-guided PIWI proteins, MIWI2, MILI, and MIWI, silence transposons, regulate expression of protein-coding genes and are necessary for fertility. A working endonuclease activity of MIWI and MILI is essential to complete spermatogenesis. Yet, both MIWI and MILI produce weak and slow target cleavage in vitro, thwarting biochemical examination of the silencing step. Here, we find that PIWI proteins require an auxiliary protein to efficiently cleave their targets, unlike any other known Argonaute. Gametocyte Specific Factor 1 (GTSF1) is a conserved zinc-finger protein essential for fertility and piRNA-directed silencing. We show GTSF1 accelerates the pre-steady-state rate of target cleavage by MIWI and MILI; this role of GTSF1 is also preserved in insects. A critical step in GTSF1 mechanism entails binding RNA. GTSF1 allowed detailed kinetic analyses of catalytic PIWIs: they require extensive 3′ complementarity between the guide and target to efficiently cleave them, but this base-pairing also limits turnover. Interestingly, within a species, different PIWI proteins have unique kinetic properties. In sum, our findings provide molecular mechanisms of GTSF1 function and target silencing by PIWIs as well as a useful method for future studies.
Arif A. (2021). Biochemical Mechanism of Gene Expression Silencing by piRNA-directed PIWI-Clade Argonautes. Morningside Graduate School of Biomedical Sciences Dissertations and Theses. https://doi.org/10.13028/e70a-dc62. Retrieved from https://escholarship.umassmed.edu/gsbs_diss/1154
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