GSBS Dissertations and Theses

ORCID ID

0000-0002-4886-5754

Publication Date

2020-06-26

Document Type

Master's Thesis

Academic Program

Neuroscience

Department

Neurobiology

First Thesis Advisor

Yang Xiang

Keywords

TrpA1, TRP Channels, Interactome, Mass Spectrometry, Co-IP, Isoforms, Nociception, Sensitization, Chronic Pain

Abstract

Nociception is the sensory nervous system that detects harmful stimuli including excessive heat, cold, toxic chemicals, and noxious mechanical stimulations. Transient receptor potential (TRP) channels are a group of evolutionarily conserved ion channels consisting of 4 subunits, each with 6 transmembrane spans, and detect a variety of external and internal nociceptive stimuli. Due to their critical roles in nociception, it is essential to understand the mechanisms that regulate TRP channels and subsequent nociception. Here, I investigated two distinct types of regulation of Drosophila transient receptor potential cation channel A1 (TrpA1): regulation via the expression of different TrpA1 isoforms, and via its binding with associated proteins. I found that one of the TrpA1 isoforms, TrpA1(E), inhibits the thermal responses of other TrpA1 isoforms in vitro. I also identified potential TrpA1 binding partners through Co- immunoprecipitation (Co-IP) and mass spectrometry analysis. These binding partners need further validation and characterization through biochemical, cellular, and behavioral assays to illustrate their roles in nociception, and may serve as potential drug targets for chronic pain.

DOI

10.13028/3mzj-kg37

Rights and Permissions

Copyright is held by the author, with all rights reserved.

Available for download on Friday, August 13, 2021

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