Title

VSL#3 probiotic treatment attenuates fibrosis without changes in steatohepatitis in a diet-induced nonalcoholic steatohepatitis model in mice

UMMS Affiliation

Department of Medicine, Division of Gastroenterology; Department of Medicine, Rheumatology Division

Publication Date

12-31-2008

Document Type

Article

Subjects

Actins; Animals; Choline Deficiency; Collagen Type I; Diet; Disease Models, Animal; Fatty Liver; Female; Liver Cirrhosis; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Methionine; Mice; Mice, Inbred C57BL; NF-kappa B; Peroxisome Proliferator-Activated Receptors; Probiotics; Signal Transduction; Transforming Growth Factor beta

Disciplines

Gastroenterology | Immunology and Infectious Disease

Abstract

Nonalcoholic fatty liver disease (NAFLD) and its advanced stage, nonalcoholic steatohepatitis (NASH), are the most common causes of chronic liver disease in the United States. NASH features the metabolic syndrome, inflammation, and fibrosis. Probiotics exhibit immunoregulatory and anti-inflammatory activity. We tested the hypothesis that probiotic VSL#3 may ameliorate the methionine-choline-deficient (MCD) diet-induced mouse model of NASH. MCD diet resulted in NASH in C57BL/6 mice compared to methionine-choline-supplemented (MCS) diet feeding evidenced by liver steatosis, increased triglycerides, inflammatory cell accumulation, increased tumor necrosis factor alpha levels, and fibrosis. VSL#3 failed to prevent MCD-induced liver steatosis or inflammation. MCD diet, even in the presence of VSL#3, induced up-regulation of serum endotoxin and expression of the Toll-like receptor 4 signaling components, including CD14 and MD2, MyD88 adaptor, and nuclear factor kappaB activation. In contrast, VSL#3 treatment ameliorated MCD diet-induced liver fibrosis resulting in diminished accumulation of collagen and alpha-smooth muscle actin. We identified increased expression of liver peroxisome proliferator-activated receptors and decreased expression of procollagen and matrix metalloproteinases in mice fed MCD+VSL#3 compared to MCD diet alone. MCD diet triggered up-regulation of transforming growth factor beta (TGFbeta), a known profibrotic agent. In the presence of VSL#3, the MCD diet-induced expression of TGFbeta was maintained; however, the expression of Bambi, a TGFbeta pseudoreceptor with negative regulatory function, was increased. In summary, our data indicate that VSL#3 modulates liver fibrosis but does not protect from inflammation and steatosis in NASH. The mechanisms of VSL#3-mediated protection from MCD diet-induced liver fibrosis likely include modulation of collagen expression and impaired TGFbeta signaling.

DOI of Published Version

10.1002/hep.22711

Source

Hepatology. 2009 Mar;49(3):989-97. Link to article on publisher's site

Journal/Book/Conference Title

Hepatology (Baltimore, Md.)

Related Resources

Link to Article in PubMed

PubMed ID

19115316

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