Regulatory potential of ethanol and retinoic acid on human monocyte functions
Department of Medicine, Division of Gastroenterology; Department of Surgery
Antigens, CD4; Cell Line; Dose-Response Relationship, Drug; Ethanol; Humans; Immune Tolerance; Monocytes; Transforming Growth Factor beta; Tretinoin; Tumor Necrosis Factor-alpha
Digestive System Diseases | Gastroenterology
Retinoic acid (RA), a metabolic product of vitamin A, has been shown to affect a variety of immune functions, including monocytes. Monocyte functions and mediator production are also modulated by ethanol exposure. This study demonstrates that therapeutic doses of RA (0.1-10 microM) significantly increase transforming growth factor-beta (TGF beta) production both in THP-1, human myelomonocytic cells, and in human peripheral blood monocytes. We have previously reported TGF beta induction by ethanol in human M theta. Combination of RA stimulation with acute in vitro ethanol treatment, however, resulted in significantly lower M theta TGF beta production than TGF beta levels induced by RA alone (p < 0.003). Down-regulation of M theta TGF beta production by ethanol was tested at the concentration range of 25-150 mM and occurred both at high and low RA concentrations (10-0.1 microM). In contrast to its inhibitory effect on RA-induced M theta TGF beta production, ethanol augmented TGF beta production induced by muramyl dipeptide (20 micrograms/ml), suggesting that ethanol can either up- or down-regulate M theta TGF beta production, depending on the costimulatory factors. RA also induced a moderate increase in M theta tumor necrosis factor-alpha (TNF alpha) production, which was down-regulated by ethanol both at the level of secreted and cell-associated TNF alpha. In addition to regulation of cytokine production, both RA and ethanol decreased expression of CD4 on THP-1 cells. The degree of inhibition of CD4 expression by RA was more significant than by ethanol, but RA-induced decrease in CD4 expression was not significantly affected by the combined stimulation with ethanol.(ABSTRACT TRUNCATED AT 250 WORDS)
Alcohol Clin Exp Res. 1994 Jun;18(3):548-54.
Alcoholism, clinical and experimental research
Szabo G, Puppolo M, Verma BK, Catalano D. (1994). Regulatory potential of ethanol and retinoic acid on human monocyte functions. Gastroenterology Publications and Presentations. Retrieved from https://escholarship.umassmed.edu/gastroenterology_pp/17