Semiconductor-based DNA sequencing of histone modification states
Program in Bioinformatics and Integrative Biology; Garber Lab
Bioinformatics | Computational Biology | Genomics | Molecular Biology
The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues.
Cancer, DNA sequencing, Epigenetics, Histone post-translational modifications
DOI of Published Version
Nat Commun. 2013;4:2672. doi: 10.1038/ncomms3672. Link to article on publisher's site
Cheng, Christine S.; Garber, Manuel; Chin, Lynda; Regev, Aviv; and Amit, Ido, "Semiconductor-based DNA sequencing of histone modification states" (2013). Garber Lab Publications. 8.