Garber Lab Publications

Title

Semiconductor-based DNA sequencing of histone modification states

UMMS Affiliation

Program in Bioinformatics and Integrative Biology; Garber Lab

Publication Date

2013-10-25

Document Type

Article

Disciplines

Bioinformatics | Computational Biology | Genomics | Molecular Biology

Abstract

The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues.

Keywords

Cancer, DNA sequencing, Epigenetics, Histone post-translational modifications

DOI of Published Version

10.1038/ncomms3672

Source

Nat Commun. 2013;4:2672. doi: 10.1038/ncomms3672. Link to article on publisher's site

Journal/Book/Conference Title

Nature communications

Comments

Full author list omitted for brevity. For the full list of authors, see article.

Related Resources

Link to Article in PubMed

PubMed ID

24157732

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