Garber Lab Publications

Title

Higher-Order Inter-chromosomal Hubs Shape 3D Genome Organization in the Nucleus

UMMS Affiliation

Program in Bioinformatics and Integrative Biology; Program in Molecular Medicine; Garber Lab

Publication Date

6-4-2018

Document Type

Article

Disciplines

Bioinformatics | Cell Biology | Computational Biology | Genetic Phenomena | Genomics | Molecular Biology | Structural Biology

Abstract

Eukaryotic genomes are packaged into a 3-dimensional structure in the nucleus. Current methods for studying genome-wide structure are based on proximity ligation. However, this approach can fail to detect known structures, such as interactions with nuclear bodies, because these DNA regions can be too far apart to directly ligate. Accordingly, our overall understanding of genome organization remains incomplete. Here, we develop split-pool recognition of interactions by tag extension (SPRITE), a method that enables genome-wide detection of higher-order interactions within the nucleus. Using SPRITE, we recapitulate known structures identified by proximity ligation and identify additional interactions occurring across larger distances, including two hubs of inter-chromosomal interactions that are arranged around the nucleolus and nuclear speckles. We show that a substantial fraction of the genome exhibits preferential organization relative to these nuclear bodies. Our results generate a global model whereby nuclear bodies act as inter-chromosomal hubs that shape the overall packaging of DNA in the nucleus.

Keywords

Nuclear structure, RNA DNA interactions, SPRITE, genome structure, higher-order nuclear structure, multi-way interactions, nuclear organization, nuclear speckle, nucleolus

DOI of Published Version

10.1016/j.cell.2018.05.024

Source

Cell. 2018 Jun 4. pii: S0092-8674(18)30636-6. doi: 10.1016/j.cell.2018.05.024. Link to article on publisher's site

Journal/Book/Conference Title

Cell

Comments

Full author list omitted for brevity. For the full list of authors, see article.

Related Resources

Link to Article in PubMed

PubMed ID

29887377

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