Garber Lab Publications

Title

Gene expression is circular: factors for mRNA degradation also foster mRNA synthesis

UMMS Affiliation

Program in Bioinformatics and Integrative Biology; Garber Lab

Publication Date

5-23-2013

Document Type

Article

Disciplines

Bioinformatics | Cell Biology | Computational Biology | Genetic Phenomena | Genomics | Molecular Biology

Abstract

Maintaining proper mRNA levels is a key aspect in the regulation of gene expression. The balance between mRNA synthesis and decay determines these levels. We demonstrate that most yeast mRNAs are degraded by the cytoplasmic 5'-to-3' pathway (the "decaysome"), as proposed previously. Unexpectedly, the level of these mRNAs is highly robust to perturbations in this major pathway because defects in various decaysome components lead to transcription downregulation. Moreover, these components shuttle between the cytoplasm and the nucleus, in a manner dependent on proper mRNA degradation. In the nucleus, they associate with chromatin-preferentially approximately 30 bp upstream of transcription start-sites-and directly stimulate transcription initiation and elongation. The nuclear role of the decaysome in transcription is linked to its cytoplasmic role in mRNA decay; linkage, in turn, seems to depend on proper shuttling of its components. The gene expression process is therefore circular, whereby the hitherto first and last stages are interconnected.

DOI of Published Version

10.1016/j.cell.2013.05.012

Source

Cell. 2013 May 23;153(5):1000-11. doi: 10.1016/j.cell.2013.05.012. Link to article on publisher's site

Journal/Book/Conference Title

Cell

Related Resources

Link to Article in PubMed

PubMed ID

23706738

Link to Full Text

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