University of Massachusetts Medical School Faculty Publications
Title
Structural basis of the relaxed state of a Ca2+-regulated myosin filament and its evolutionary implications
UMMS Affiliation
Department of Cell and Development Biology
Publication Date
2013-05-21
Document Type
Article
Subjects
Calcium-Binding Proteins; Myosins
Disciplines
Cell and Developmental Biology | Cell Biology
Abstract
Myosin filaments of muscle are regulated either by phosphorylation of their regulatory light chains or Ca(2+) binding to the essential light chains, contributing to on-off switching or modulation of contraction. Phosphorylation-regulated filaments in the relaxed state are characterized by an asymmetric interaction between the two myosin heads, inhibiting their actin binding or ATPase activity. Here, we have tested whether a similar interaction switches off activity in myosin filaments regulated by Ca(2+) binding. Cryo-electron microscopy and single-particle image reconstruction of Ca(2+)-regulated (scallop) filaments reveals a helical array of myosin head-pair motifs above the filament surface. Docking of atomic models of scallop myosin head domains into the motifs reveals that the heads interact in a similar way to those in phosphorylation-regulated filaments. The results imply that the two major evolutionary branches of myosin regulation-involving phosphorylation or Ca(2+) binding-share a common structural mechanism for switching off thick-filament activity in relaxed muscle. We suggest that the Ca(2+)-binding mechanism evolved from the more ancient phosphorylation-based system to enable rapid response of myosin-regulated muscles to activation. Although the motifs are similar in both systems, the scallop structure is more tilted and higher above the filament backbone, leading to different intermolecular interactions. The reconstruction reveals how the myosin tail emerges from the motif, connecting the heads to the filament backbone, and shows that the backbone is built from supramolecular assemblies of myosin tails. The reconstruction provides a native structural context for understanding past biochemical and biophysical studies of this model Ca(2+)-regulated myosin.
DOI of Published Version
10.1073/pnas.1218462110
Source
Proc Natl Acad Sci U S A. 2013 May 21;110(21):8561-6. doi: 10.1073/pnas.1218462110. Link to article on publisher's site
Related Resources
Journal/Book/Conference Title
Proceedings of the National Academy of Sciences of the United States of America
PubMed ID
23650385
Repository Citation
Woodhead JL, Zhao F, Craig RW. (2013). Structural basis of the relaxed state of a Ca2+-regulated myosin filament and its evolutionary implications. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1073/pnas.1218462110. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/9