University of Massachusetts Medical School Faculty Publications
UMMS Affiliation
Department of Cell and Developmental Biology
Publication Date
2014-01-01
Document Type
Article
Subjects
Amino Acid Sequence; Axoneme; Chlamydomonas reinhardtii; Conserved Sequence; Cytoplasm; Enzyme Stability; Flagella; Peptide Synthases; Plant Proteins; Protein Binding; Protein Processing, Post-Translational; Protein Transport; Sequence Homology, Amino Acid; Tubulin
Disciplines
Cell Biology | Molecular Biology
Abstract
Tubulin undergoes various posttranslational modifications, including polyglutamylation, which is catalyzed by enzymes belonging to the tubulin tyrosine ligase-like protein (TTLL) family. A previously isolated Chlamydomonas reinhardtii mutant, tpg1, carries a mutation in a gene encoding a homologue of mammalian TTLL9 and displays lowered motility because of decreased polyglutamylation of axonemal tubulin. Here we identify a novel tpg1-like mutant, tpg2, which carries a mutation in the gene encoding FAP234, a flagella-associated protein of unknown function. Immunoprecipitation and sucrose density gradient centrifugation experiments show that FAP234 and TTLL9 form a complex. The mutant tpg1 retains FAP234 in the cell body and flagellar matrix but lacks it in the axoneme. In contrast, tpg2 lacks both TTLL9 and FAP234 in all fractions. In fla10, a temperature-sensitive mutant deficient in intraflagellar transport (IFT), both TTLL9 and FAP234 are lost from the flagellum at nonpermissive temperatures. These and other results suggest that FAP234 functions in stabilization and IFT-dependent transport of TTLL9. Both TTLL9 and FAP234 are conserved in most ciliated organisms. We propose that they constitute a polyglutamylation complex specialized for regulation of ciliary motility.
Rights and Permissions
© 2014 Kubo et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0)
DOI of Published Version
10.1091/mbc.E13-07-0424
Source
Mol Biol Cell. 2014 Jan;25(1):107-17. doi: 10.1091/mbc.E13-07-0424. Epub 2013 Nov 6. Link to article on publisher's site
Related Resources
Journal/Book/Conference Title
Molecular biology of the cell
PubMed ID
24196831
Repository Citation
Kubo T, Yanagisawa H, Liu Z, Shibuya R, Hirono M, Kamiya R. (2014). A conserved flagella-associated protein in Chlamydomonas, FAP234, is essential for axonemal localization of tubulin polyglutamylase TTLL9. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1091/mbc.E13-07-0424. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/824
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.