Department of Medicine, Division of Infectious Diseases and Immunology
Animals; Biolistics; Electroporation; Humans; Immunization; Injections; Vaccines, DNA
Immunoprophylaxis and Therapy
DNA immunization was discovered in early 1990s, and its use has been expanded from vaccine studies to a broader range of biomedical research areas, such as the generation of high-quality polyclonal and monoclonal antibodies as research reagents. In this unit, three common DNA immunization methods are described: needle injection, electroporation, and gene gun. In addition, several common considerations related to DNA immunization are discussed.
DNA vaccine, electroporation, gene gun, immunization
DOI of Published Version
Curr Protoc Microbiol. 2013 Nov 5;31:18.3.1-18.3.24. doi:10.1002/9780471729259.mc1803s31. Link to article on publisher's site
Current protocols in microbiology
Wang, Shixia and Lu, Shan, "DNA immunization" (2013). University of Massachusetts Medical School Faculty Publications. 790.