Loss of cilia suppresses cyst growth in genetic models of autosomal dominant polycystic kidney disease
Program in Molecular Medicine
Animals; Cilia; Cysts; Disease Models, Animal; Genotype; Mice; Mice, Knockout; Mutation; Phenotype; Polycystic Kidney, Autosomal Dominant; TRPP Cation Channels
Kidney cysts occur following inactivation of polycystins in otherwise intact cilia or following complete removal of cilia by inactivation of intraflagellar transport-related proteins. We investigated the mechanisms of cyst formation in these two distinct processes by combining conditional inactivation of polycystins with concomitant ablation of cilia in developing and adult kidney and liver. We found that loss of intact cilia suppressed cyst growth following inactivation of polycystins and that the severity of cystic disease was directly related to the length of time between the initial loss of the polycystin proteins and the subsequent involution of cilia. This cilia-dependent cyst growth was not explained by activation of the MAPK/ERK, mTOR or cAMP pathways and is likely to be distinct from the mechanism of cyst growth following complete loss of cilia. These data establish the existence of a new pathway defined by polycystin-dependent inhibition and cilia-dependent activation that promotes rapid cyst growth.
DOI of Published Version
Nat Genet. 2013 Sep;45(9):1004-12. doi: 10.1038/ng.2715. Epub 2013 Jul 28. Link to article on publisher's site
Ma, Ming; Tian, Xin; Igarashi, Peter; Pazour, Gregory J.; and Somlo, Stefan, "Loss of cilia suppresses cyst growth in genetic models of autosomal dominant polycystic kidney disease" (2013). University of Massachusetts Medical School Faculty Publications. 585.