Quantification of nanosized extracellular membrane vesicles with scanning ion occlusion sensing
Authors
de Vrij, JeroenMaas, Sybren L.N.
van Nispen, Malisa
Sena-Esteves, Miguel
Limpens, Ronald W.A.
Koster, Abraham J.
Leenstra, Sieger
Lamfers, Martine L.
Broekman, Marike L. D
UMass Chan Affiliations
Department of Neurology, Gene Therapy CenterDocument Type
Journal ArticlePublication Date
2013-09-01Keywords
Biological TransportBody Fluids
Humans
Ions
Membranes
*Nanopores
Particle Size
Secretory Vesicles
Nanomedicine
Metadata
Show full item recordAbstract
BACKGROUND: Cells secrete different types of membrane vesicles (MVs), which may act as important entities in normal human physiology and in various pathological processes. The established methods for quantification of MVs require purification or preanalytical handling of samples with labeling moieties. AIM: The authors' aim was to develop a method for high-throughput, labeling-free quantification of nonpurified MVs. MATERIALS and METHODS: Scanning ion occlusion sensing technology, which relies on the detection of particles upon their movement through a nanopore, was investigated for the ability to quantify nanosized MVs ( < 400 nm) in bodily fluids and cell culture supernatants. RESULTS: Scanning ion occlusion sensing allowed for rapid and easy measurement of the concentration of MVs in all biological fluids tested. CONCLUSION: Scanning ion occlusion sensing technology enables the quantification of MVs in biological samples without the requirement of MV isolation and/or labeling. This offers a highly valuable addition to the currently used repertoire of MV quantification methods.Source
Nanomedicine (Lond). 2013 Sep;8(9):1443-58. doi: 10.2217/nnm.12.173. Epub 2013 Feb 5. Link to article on publisher's site
DOI
10.2217/nnm.12.173Permanent Link to this Item
http://hdl.handle.net/20.500.14038/30308PubMed ID
23384702Related Resources
ae974a485f413a2113503eed53cd6c53
10.2217/nnm.12.173