Post-translational modification by cysteine protects Cu/Zn-superoxide dismutase from oxidative damage
Department of Neurology
Amino Acid Sequence; Animals; Brain; Cysteine; Humans; Hydrogen Peroxide; Mice; Oxidation-Reduction; Oxygen; Protein Processing, Post-Translational; Spinal Cord; Superoxide Dismutase
Reactive oxygen species (ROS) are cytotoxic. To remove ROS, cells have developed ROS-specific defense mechanisms, including the enzyme Cu/Zn superoxide dismutase (SOD1), which catalyzes the disproportionation of superoxide anions into molecular oxygen and hydrogen peroxide. Although hydrogen peroxide is less reactive than superoxide, it is still capable of oxidizing, unfolding, and inactivating SOD1, at least in vitro. To explore the relevance of post-translational modification (PTM) of SOD1, including peroxide-related modifications, SOD1 was purified from postmortem human nervous tissue. As much as half of all purified SOD1 protein contained non-native post-translational modifications (PTMs), the most prevalent modifications being cysteinylation and peroxide-related oxidations. Many PTMs targeted a single reactive SOD1 cysteine, Cys111. An intriguing observation was that unlike native SOD1, cysteinylated SOD1 was not oxidized. To further characterize how cysteinylation may protect SOD1 from oxidation, cysteine-modified SOD1 was prepared in vitro and exposed to peroxide. Cysteinylation conferred nearly complete protection from peroxide-induced oxidation of SOD1. Moreover, SOD1 that has been cysteinylated and peroxide oxidized in vitro comprised a set of PTMs that bear a striking resemblance to the myriad of PTMs observed in SOD1 purified from human tissue.
DOI of Published Version
Biochemistry. 2013 Sep 10;52(36):6137-44. doi: 10.1021/bi4006122. Epub 2013 Aug 26. Link to article on publisher's site
Auclair, Jared R.; Johnson, Joshua L.; Liu, Qian; Salisbury, Joseph P.; Rotunno, Melissa S.; Petsko, Gregory A.; Ringe, Dagmar; Brown, Robert H. Jr.; Bosco, Daryl A.; and Agar, Jeffrey N., "Post-translational modification by cysteine protects Cu/Zn-superoxide dismutase from oxidative damage" (2013). University of Massachusetts Medical School Faculty Publications. 556.