Detection of necrosis by release of lactate dehydrogenase activity
Diabetes and Endocrinology Research Center; Department of Pathology
Cell and Developmental Biology | Cell Biology | Diagnosis | Laboratory and Basic Science Research | Pathological Conditions, Signs and Symptoms | Pathology
Apoptosis and necrosis are two major forms of cell death observed in normal and disease pathologies. Although there are many assays for detection of apoptosis, relatively few assays are available for measuring necrosis. A key signature for necrotic cells is the permeabilization of the plasma membrane. This event can be quantified in tissue culture settings by measuring the release of the intracellular enzyme lactate dehydrogenase (LDH). When combined with other methods, measuring LDH release is a useful method for the detection of necrosis. In this chapter, we describe the step-by-step procedure for detection of LDH release from necrotic cells using a microtiter plate-based colorimetric absorbance assay.
DOI of Published Version
Methods Mol Biol. 2013;979:65-70. doi: 10.1007/978-1-62703-290-2_7. Link to article on publisher's site
Methods in molecular biology (Clifton, N.J.)
Chan, Francis Ka-Ming; Moriwaki, Kenta; and De Rosa, Maria Jose, "Detection of necrosis by release of lactate dehydrogenase activity" (2013). University of Massachusetts Medical School Faculty Publications. 236.