UMass Chan Medical School Faculty Publications


Analysis of ciliary membrane protein dynamics using SNAP technology

UMMS Affiliation

Program in Molecular Medicine

Publication Date


Document Type

Book Chapter


Cell and Developmental Biology | Cellular and Molecular Physiology | Molecular Biology


The sensory functions of the primary cilium rely on receptors and other membrane proteins that are specifically sorted to the ciliary compartment, which is a subdomain of the plasma membrane. Defects in this process underlie a large number of human diseases, yet it is poorly understood. Thus, it is of great interest to understand the mechanisms by which the cell sorts and traffics proteins to the ciliary membrane. Here, we provide an overview of our method to study the sorting and trafficking of ciliary membrane proteins using SNAP technology. This technology enables pulse-chase analysis of the movement of proteins through the endomembrane system and onto the cilium.

DOI of Published Version



Methods Enzymol. 2013;524:195-204. doi: 10.1016/B978-0-12-397945-2.00011-1. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Methods in enzymology

PubMed ID