Department of Biochemistry and Molecular Pharmacology
Amino Acids, Peptides, and Proteins | Fungi | Molecular Biology
Hsp70 interactions are critical for cellular viability and the response to stress. Previous attempts to characterize Hsp70 interactions have been limited by their transient nature and inability of current technologies to distinguish direct vs bridged interactions. We report the novel use of cross-linking mass spectrometry (XL-MS) to comprehensively characterize the budding yeast Hsp70 protein interactome. Using this approach, we have gained fundamental new insights into Hsp70 function, including definitive evidence of Hsp70 self-association as well as multi-point interaction with its client proteins. In addition to identifying a novel set of direct Hsp70 interactors which can be used to probe chaperone function in cells, we have also identified a suite of PTM-associated Hsp70 interactions. The majority of these PTMs have not been previously reported and appear to be critical in the regulation of client protein function. These data indicate that one of the mechanisms by which PTMs contribute to protein function is by facilitating interaction with chaperones. Taken together, we propose that XL-MS analysis of chaperone complexes may be used as a unique way to identify biologically-important PTMs on client proteins.
In vivo confirmation of Hsp70 dimerization
Comprehensive direct interactome of Hsp70
Multi-domain interactions between Hsp70 and client proteins
Identification of novel biologically-important client protein PTMs
Hsp70, Ssa1, chaperone, cross-linking, mass spectrometry, posttranslational modifications, interactions, proteomics
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DOI of Published Version
bioRxiv 2021.08.25.457671; doi: https://doi.org/10.1101/2021.08.25.457671. Link to preprint on bioRxiv.
Nitika, Winters MJ, Pryciak PM. (2021). A novel multifunctional role for Hsp70 in binding post-translational modifications on client proteins [preprint]. UMass Chan Medical School Faculty Publications. https://doi.org/10.1101/2021.08.25.457671. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/2105
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