UMass Chan Medical School Faculty Publications

UMMS Affiliation

Program in Molecular Medicine

Publication Date

2021-01-27

Document Type

Article Preprint

Disciplines

Biochemistry, Biophysics, and Structural Biology | Bioimaging and Biomedical Optics | Bioinformatics | Laboratory and Basic Science Research | Research Methods in Life Sciences

Abstract

In April 2020, the QUality Assessment and REProducibility for Instruments and Images in Light Microscopy (QUAREP-LiMi) initiative was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved quality control (QC) in light microscopy. The ultimate goal of the QUAREP-LiMi initiative is to establish a set of common QC standards, guidelines, metadata models, and tools, including detailed protocols, with the ultimate aim of improving reproducible advances in scientific research. This White Paper 1) summarizes the major obstacles identified in the field that motivated the launch of the QUAREP-LiMi initiative; 2) identifies the urgent need to address these obstacles in a grassroots manner, through a community of stakeholders including, researchers, imaging scientists, bioimage analysts, bioimage informatics developers, corporate partners, funding agencies, standards organizations, scientific publishers, and observers of such; 3) outlines the current actions of the QUAREP-LiMi initiative, and 4) proposes future steps that can be taken to improve the dissemination and acceptance of the proposed guidelines to manage QC. To summarize, the principal goal of the QUAREP-LiMi initiative is to improve the overall quality and reproducibility of light microscope image data by introducing broadly accepted standard practices and accurately captured image data metrics.

Keywords

light microscopy, reproducibility, standards, quality, reproducibility

Rights and Permissions

Distributed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license.

DOI of Published Version

10.1111/jmi.13041

Source

Nelson G, Boehm U, Bagley S, Bajcsy P, Bischof J, Brown CM, Dauphin A, Dobbie IM, Eriksson JE, Faklaris O, Fernandez-Rodriguez J. QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy. arXiv preprint arXiv:2101.09153. 2021 Jan 21. Link to preprint on arXiv.

Comments

This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.

The PDF available for download is Version 2 of this preprint. The complete version history of this preprint is available at arXiv.org.

Full author list omitted for brevity. For the full list of authors, see article.

Related Resources

Now published in Journal of Microscopy, doi:10.1111/jmi.13041.

Journal/Book/Conference Title

arXiv

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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