University of Massachusetts Medical School Faculty Publications
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology; Department of Neurology; RNA Therapeutics Institute
Publication Date
2021-08-04
Document Type
Article Preprint
Disciplines
Nervous System Diseases | Neurology | Neuroscience and Neurobiology
Abstract
Modestly increased expression of transactive response DNA binding protein (TDP-43) gene have been reported in amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), and other neuromuscular diseases. However, whether this modest elevation triggers neurodegeneration is not known. Although high levels of TDP-43 overexpression have been modeled in mice and shown to cause early death, models with low-level overexpression that mimic the human condition have not been established. In this study, transgenic mice overexpressing wild type TDP-43 at less than 60% above the endogenous CNS levels were constructed, and their phenotypes analyzed by a variety of techniques, including biochemical, molecular, histological, behavioral techniques and electromyography. The TDP-43 transgene was expressed in neurons, astrocytes, and oligodendrocytes in the cortex and predominantly in astrocytes and oligodendrocytes in the spinal cord. The mice developed a reproducible progressive weakness ending in paralysis in mid-life. Detailed analysis showed ∼30% loss of large pyramidal neurons in the layer V motor cortex; in the spinal cord, severe demyelination was accompanied by oligodendrocyte injury, protein aggregation, astrogliosis and microgliosis, and elevation of neuroinflammation. Surprisingly, there was no loss of lower motor neurons in the lumbar spinal cord despite the complete paralysis of the hindlimbs. However, denervation was detected at the neuromuscular junction. These results demonstrate that low-level TDP-43 overexpression can cause diverse aspects of ALS, including late-onset and progressive motor dysfunction, neuroinflammation, and neurodegeneration. Our findings suggest that persistent modest elevations in TDP-43 expression can lead to ALS and other neurological disorders involving TDP-43 proteinopathy. Because of the predictable and progressive clinical paralytic phenotype, this transgenic mouse model will be useful in preclinical trial of therapeutics targeting neurological disorders associated with elevated levels of TDP-43.
Keywords
transactive response DNA binding protein (TDP-43) gene, neuromuscular diseases, neurodegeneration, therapeutics
Rights and Permissions
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license.
DOI of Published Version
10.1101/2021.08.04.455119
Source
bioRxiv 2021.08.04.455119; doi: https://doi.org/10.1101/2021.08.04.455119. Link to preprint on bioRxiv.
Journal/Book/Conference Title
bioRxiv
Repository Citation
Yang C, Qiao T, Yu J, Wang H, Guo Y, Salameh J, Metterville JP, Parsi S, Brown RH, Cai H, Xu Z. (2021). Low-level overexpression of wild type TDP-43 causes late-onset, progressive neurodegeneration and paralysis in mice [preprint]. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1101/2021.08.04.455119. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/2083
Creative Commons License
This work is licensed under a Creative Commons 1.0 Public Domain Dedication.
Included in
Nervous System Diseases Commons, Neurology Commons, Neuroscience and Neurobiology Commons
Comments
This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.