Program in Molecular Medicine; Department of Microbiology and Physiological Systems; Graduate School of Biomedical Sciences
Amino Acids, Peptides, and Proteins | Cell Biology | Cellular and Molecular Physiology | Congenital, Hereditary, and Neonatal Diseases and Abnormalities | Enzymes and Coenzymes | Female Urogenital Diseases and Pregnancy Complications | Genetics and Genomics | Male Urogenital Diseases
Polycystic kidney disease is an inherited degenerative disease in which the uriniferous tubules are replaced by expanding fluid-filled cysts that ultimately destroy organ function. Autosomal dominant polycystic kidney disease (ADPKD) is the most common form, afflicting approximately 1 in 1,000 people. It primarily is caused by mutations in the transmembrane proteins polycystin-1 (Pkd1) and polycystin-2 (Pkd2). The most proximal effects of Pkd mutations leading to cyst formation are not known, but pro-proliferative signaling must be involved for the tubule epithelial cells to increase in number over time. The c-Jun N-terminal kinase (JNK) pathway promotes proliferation and is activated in acute and chronic kidney diseases. Using a mouse model of cystic kidney disease caused by Pkd2 loss, we observe JNK activation in cystic kidneys and observe increased nuclear phospho c-Jun in cystic epithelium. Genetic removal of Jnk1 and Jnk2 suppresses the nuclear accumulation of phospho c-Jun, reduces proliferation and reduces the severity of cystic disease. While Jnk1 and Jnk2 are thought to have largely overlapping functions, we find that Jnk1 loss is nearly as effective as the double loss of Jnk1 and Jnk2. Jnk pathway inhibitors are in development for neurodegeneration, cancer, and fibrotic diseases. Our work suggests that the JNK pathway should be explored as a therapeutic target for ADPKD.
Genetics, autosomal dominant polycystic kidney disease, mutations, c-Jun N-terminal kinase (JNK) pathway
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DOI of Published Version
bioRxiv 2021.07.15.452451; doi: https://doi.org/10.1101/2021.07.15.452451. Link to preprint on bioRxiv.
Smith AO, Jonassen JA, Preval K, Davis RJ, Pazour GJ. (2021). c-Jun N-terminal kinase (JNK) signaling contributes to cystic burden in polycystic kidney disease [preprint]. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1101/2021.07.15.452451. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/2078
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Amino Acids, Peptides, and Proteins Commons, Cell Biology Commons, Cellular and Molecular Physiology Commons, Congenital, Hereditary, and Neonatal Diseases and Abnormalities Commons, Enzymes and Coenzymes Commons, Female Urogenital Diseases and Pregnancy Complications Commons, Genetics and Genomics Commons, Male Urogenital Diseases Commons