The testis-specific transcription factor TCFL5 responds to A-MYB to elaborate the male meiotic program in placental mammals [preprint]
Authors
Ozata, Deniz MYu, Tianxiong
Cecchini, Katharine
Mou, Haiwei
Arif, Amena
Colpan, Cansu
Biasini, Adriano
Gaitendinov, Ildar
de Rooij, Dirk G.
Weng, Zhiping
Zamore, Phillip D.
UMass Chan Affiliations
Graduate School of Biomedical SciencesProgram in Bioinformatics and Integrative Biology
RNA Therapeutics Institute
Document Type
PreprintPublication Date
2021-04-05Keywords
Geneticstranscription factors
meiosis
spermiogenesis
spermatogenesis
Cell and Developmental Biology
Genetics and Genomics
Metadata
Show full item recordAbstract
In male mice, the transcription factor (TF) A-MYB initiates reprogramming of gene expression after spermatogonia enter meiosis. We report that A-MYB activates Tcfl5, a testis-specific TF first produced in pachytene spermatocytes. Subsequently, A-MYB and TCFL5 reciprocally reinforce their own transcription to establish an extensive circuit that regulates meiosis. TCFL5 promotes transcription of genes required for mRNA turnover, pachytene piRNA production, meiotic exit, and spermiogenesis. This transcriptional architecture is conserved in rhesus macaque, suggesting TCFL5 plays a central role in meiosis and spermiogenesis in placental mammals. Tcfl5em1/em1 mutants are sterile, and spermatogenesis arrests at the mid- or late-pachytene stage of meiosis.Source
bioRxiv 2021.04.04.438419; doi: https://doi.org/10.1101/2021.04.04.438419. Link to preprint on bioRxiv.
DOI
10.1101/2021.04.04.438419Permanent Link to this Item
http://hdl.handle.net/20.500.14038/29816Notes
This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.
Rights
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.Distribution License
http://creativecommons.org/licenses/by-nc-nd/4.0/ae974a485f413a2113503eed53cd6c53
10.1101/2021.04.04.438419
Scopus Count
Except where otherwise noted, this item's license is described as The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.