Department of Biochemistry and Molecular Pharmacology; Cryo-EM Core Facility
Biomedical Engineering and Bioengineering
FastTomo is a SerialEM script for collecting tilted specimen images in transmission electron microscopes to be further used in tomographic reconstruction. It achieves a speedup over conventional tracking methods by minimizing the usage of off-target tracking shots, and instead applies proportional control to the specimen images. Movement in the Z coordinate is estimated prior to each tilt series in a separate calibration routine. Overall, this method is fast and reliable when the field of view is at least 1 um, and can tolerate minor errors in setting eucentric height. The implemented tilt series schemes include the unidirectional, bidirectional, and dose-symmetric schemes.
Bioengineering, FastTomo, electron tomography, cryoET, eucentricity, proportional control, pre-calibration
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DOI of Published Version
bioRxiv 2021.03.16.435675; doi: https://doi.org/10.1101/2021.03.16.435675. Link to preprint on bioRxiv.
Xu A, Xu C. (2021). FastTomo: A SerialEM Script for Collecting Electron Tomography Data [preprint]. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1101/2021.03.16.435675. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/2022
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