University of Massachusetts Medical School Faculty Publications
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology; Cryo-EM Core Facility
Publication Date
2021-03-17
Document Type
Article Preprint
Disciplines
Biomedical Engineering and Bioengineering
Abstract
FastTomo is a SerialEM script for collecting tilted specimen images in transmission electron microscopes to be further used in tomographic reconstruction. It achieves a speedup over conventional tracking methods by minimizing the usage of off-target tracking shots, and instead applies proportional control to the specimen images. Movement in the Z coordinate is estimated prior to each tilt series in a separate calibration routine. Overall, this method is fast and reliable when the field of view is at least 1 um, and can tolerate minor errors in setting eucentric height. The implemented tilt series schemes include the unidirectional, bidirectional, and dose-symmetric schemes.
Keywords
Bioengineering, FastTomo, electron tomography, cryoET, eucentricity, proportional control, pre-calibration
Rights and Permissions
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
DOI of Published Version
10.1101/2021.03.16.435675
Source
bioRxiv 2021.03.16.435675; doi: https://doi.org/10.1101/2021.03.16.435675. Link to preprint on bioRxiv.
Journal/Book/Conference Title
bioRxiv
Repository Citation
Xu A, Xu C. (2021). FastTomo: A SerialEM Script for Collecting Electron Tomography Data [preprint]. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1101/2021.03.16.435675. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/2022
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Comments
This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.