UMass Chan Medical School Faculty Publications

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Department of Biochemistry and Molecular Pharmacology; Cryo-EM Core Facility

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Article Preprint


Biomedical Engineering and Bioengineering


FastTomo is a SerialEM script for collecting tilted specimen images in transmission electron microscopes to be further used in tomographic reconstruction. It achieves a speedup over conventional tracking methods by minimizing the usage of off-target tracking shots, and instead applies proportional control to the specimen images. Movement in the Z coordinate is estimated prior to each tilt series in a separate calibration routine. Overall, this method is fast and reliable when the field of view is at least 1 um, and can tolerate minor errors in setting eucentric height. The implemented tilt series schemes include the unidirectional, bidirectional, and dose-symmetric schemes.


Bioengineering, FastTomo, electron tomography, cryoET, eucentricity, proportional control, pre-calibration

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DOI of Published Version



bioRxiv 2021.03.16.435675; doi: Link to preprint on bioRxiv.


This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.

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Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.