University of Massachusetts Medical School Faculty Publications

UMMS Affiliation

Program in Molecular Medicine

Publication Date

2021-04-29

Document Type

Article Preprint

Disciplines

Amino Acids, Peptides, and Proteins | Bacteria | Bacterial Infections and Mycoses | Bacteriology | Pathogenic Microbiology

Abstract

Pseudomonas aeruginosa PA14, an opportunistic pathogen of diverse hosts, contains genes with the potential to confer production of R-bodies (i.e., a “reb cluster”). R-bodies are large, extendable protein polymers best known for their role in killing of paramecia by the bacterium Caedibacter taeniospiralis, and genes in the reb cluster have been implicated in PA14 virulence. Here, we present evidence that PA14 expresses reb cluster genes during colonization of plant and nematode hosts. We identify products of the reb cluster that are R-body-associated and that control stochastic expression of R-body structural genes. We also show that R-body production is required for full virulence in nematodes. Analyses of nematode ribosome content and immune response indicate that R-bodies act via a mechanism involving ribosome cleavage and translational inhibition. These observations provide insight into the biology of R-body production and its consequences during P. aeruginosa infection.

Keywords

Microbiology, Pseudomonas aeruginosa, protein polymers, R-bodies

Rights and Permissions

The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.

DOI of Published Version

10.1101/2020.10.26.356394

Source

bioRxiv 2020.10.26.356394; doi: https://doi.org/10.1101/2020.10.26.356394. Link to preprint on bioRxiv.

Comments

This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.

The PDF available for download is Version 3 of this preprint. The complete version history of this preprint is available at bioRxiv.

Journal/Book/Conference Title

bioRxiv

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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