University of Massachusetts Medical School Faculty Publications

UMMS Affiliation

Department of Medicine

Publication Date

2021-01-29

Document Type

Article Preprint

Disciplines

Amino Acids, Peptides, and Proteins | Diagnosis | Genetics and Genomics | Health Services Administration | Immunology and Infectious Disease | Infectious Disease | International Public Health | Parasitic Diseases | Parasitology | Population Biology

Abstract

Malaria diagnostic testing in Africa is threatened by Plasmodium falciparum parasites lacking histidine-rich protein 2 (pfhrp2) and 3 (pfhrp3) genes. Among 12,572 subjects enrolled along Ethiopia’s borders with Eritrea, Sudan, and South Sudan and using multiple assays, we estimate HRP2-based rapid diagnostic tests would miss 9.7% (95% CI 8.5-11.1) of falciparum malaria cases due to pfhrp2 deletion. Established and novel genomic tools reveal distinct subtelomeric deletion patterns, well-established pfhrp3 deletions, and recent expansion of pfhrp2 deletion. Current diagnostic strategies need to be urgently reconsidered in Ethiopia, and expanded surveillance is needed throughout the Horn of Africa.

Keywords

Infectious Diseases, hrp2, hrp3, malaria, molecular inversion probe, genomics, deletion, evolution, Ethiopia

Rights and Permissions

The copyright holder for this preprint is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.

DOI of Published Version

10.1101/2021.01.26.21250503

Source

medRxiv 2021.01.26.21250503; doi: https://doi.org/10.1101/2021.01.26.21250503. Link to preprint on medRxiv

Comments

This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.

Full author list omitted for brevity. For the full list of authors, see article.

Journal/Book/Conference Title

medRxiv

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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