University of Massachusetts Medical School Faculty Publications
UMMS Affiliation
Department of Medicine
Publication Date
2021-01-29
Document Type
Article Preprint
Disciplines
Amino Acids, Peptides, and Proteins | Diagnosis | Genetics and Genomics | Health Services Administration | Immunology and Infectious Disease | Infectious Disease | International Public Health | Parasitic Diseases | Parasitology | Population Biology
Abstract
Malaria diagnostic testing in Africa is threatened by Plasmodium falciparum parasites lacking histidine-rich protein 2 (pfhrp2) and 3 (pfhrp3) genes. Among 12,572 subjects enrolled along Ethiopia’s borders with Eritrea, Sudan, and South Sudan and using multiple assays, we estimate HRP2-based rapid diagnostic tests would miss 9.7% (95% CI 8.5-11.1) of falciparum malaria cases due to pfhrp2 deletion. Established and novel genomic tools reveal distinct subtelomeric deletion patterns, well-established pfhrp3 deletions, and recent expansion of pfhrp2 deletion. Current diagnostic strategies need to be urgently reconsidered in Ethiopia, and expanded surveillance is needed throughout the Horn of Africa.
Keywords
Infectious Diseases, hrp2, hrp3, malaria, molecular inversion probe, genomics, deletion, evolution, Ethiopia
Rights and Permissions
The copyright holder for this preprint is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
DOI of Published Version
10.1101/2021.01.26.21250503
Source
medRxiv 2021.01.26.21250503; doi: https://doi.org/10.1101/2021.01.26.21250503. Link to preprint on medRxiv
Journal/Book/Conference Title
medRxiv
Repository Citation
Feleke SM, Hathaway NJ, Cunningham J, Parr JB. (2021). Emergence and evolution of Plasmodium falciparum histidine-rich protein 2 and 3 deletion mutant parasites in Ethiopia [preprint]. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1101/2021.01.26.21250503. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1922
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Included in
Amino Acids, Peptides, and Proteins Commons, Diagnosis Commons, Genetics and Genomics Commons, Health Services Administration Commons, Infectious Disease Commons, International Public Health Commons, Parasitic Diseases Commons, Parasitology Commons, Population Biology Commons
Comments
This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.
Full author list omitted for brevity. For the full list of authors, see article.