Program in Molecular Medicine; Graduate School of Biomedical Sciences
Bioinformatics | Genetics and Genomics | Molecular Biology
Here we describe a rRNA depletion protocol based on RNase H digestion using antisense oligonucleotides (ASOs) specifically designed for C. elegans cytoplasmic rRNA (Fig. 1A). We suggest that this rRNA depletion protocol is applicable to RNA-seq applications where the yield of mRNA enrichment should be independent of poly(A) status, or any application which benefits from the removal of rRNA sequences, such ribosome profiling, or sequencing of non-coding RNAs other than rRNA.
ribosomal RNA, rRNA depletion, mRNA, RNA-seq
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DOI of Published Version
Duan Y, Sun Y, Ambros V. RNA-seq with RNase H-based ribosomal RNA depletion specifically designed for C. elegans. MicroPubl Biol. 2020 Sep 22;2020:10.17912/micropub.biology.000312. doi: 10.17912/micropub.biology.000312. PMID: 33005886; PMCID: PMC7520128. Link to article on publisher's site
Duan Y, Sun Y, Ambros VR. (2020). RNA-seq with RNase H-based ribosomal RNA depletion specifically designed for C. elegans. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.17912/micropub.biology.000312. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1901
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This work is licensed under a Creative Commons Attribution 4.0 License.