UMass Chan Medical School Faculty Publications
UMMS Affiliation
Program in Molecular Medicine; Graduate School of Biomedical Sciences
Publication Date
2020-09-22
Document Type
Article
Disciplines
Bioinformatics | Genetics and Genomics | Molecular Biology
Abstract
Here we describe a rRNA depletion protocol based on RNase H digestion using antisense oligonucleotides (ASOs) specifically designed for C. elegans cytoplasmic rRNA (Fig. 1A). We suggest that this rRNA depletion protocol is applicable to RNA-seq applications where the yield of mRNA enrichment should be independent of poly(A) status, or any application which benefits from the removal of rRNA sequences, such ribosome profiling, or sequencing of non-coding RNAs other than rRNA.
Keywords
ribosomal RNA, rRNA depletion, mRNA, RNA-seq
Rights and Permissions
Copyright: © 2020 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI of Published Version
10.17912/micropub.biology.000312
Source
Duan Y, Sun Y, Ambros V. RNA-seq with RNase H-based ribosomal RNA depletion specifically designed for C. elegans. MicroPubl Biol. 2020 Sep 22;2020:10.17912/micropub.biology.000312. doi: 10.17912/micropub.biology.000312. PMID: 33005886; PMCID: PMC7520128. Link to article on publisher's site
Related Resources
Journal/Book/Conference Title
microPublication biology
PubMed ID
33005886
Repository Citation
Duan Y, Sun Y, Ambros VR. (2020). RNA-seq with RNase H-based ribosomal RNA depletion specifically designed for C. elegans. UMass Chan Medical School Faculty Publications. https://doi.org/10.17912/micropub.biology.000312. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1901
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.