University of Massachusetts Medical School Faculty Publications

UMMS Affiliation

Program in Molecular Medicine; Graduate School of Biomedical Sciences

Publication Date

2020-10-08

Document Type

Article

Disciplines

Amino Acids, Peptides, and Proteins | Bioinformatics | Biological Engineering | Genetics and Genomics | Molecular Biology

Abstract

Here, we describe a platformed “jump board” strategy and its application in systematically engineering the essential microRNA let-7 (Fig. 1A-E) and protein coding gene lin-28 (Fig. 1F) in C. elegans. We chose the jump board protospacer sequence (INPP4A) which is (1) comprised of a PAM site and a protospacer antisense to a crRNA with experimentally confirmed high editing efficiency (INPP4A-crRNA), and (2) non-homologous to C. elegans genome, including the genetic balancer we used (mnDp1). Notably, the jump board protospacer contains an EcoRV restriction site, which can be utilized for rapid large-scale genotyping by which HDR events can be identified in the F1 generation (Fig. 1C). Using the jump board strategy, we have so far created 28 let-7 alleles for various experimental purposes, among which 15 alleles showed lethality and require rescue by mnDp1. Note that the let-7 jump board allele (ma393) itself is a new let-7 null allele in which the precursor-let-7 is completely removed.

Keywords

CRISPR/Cas9 genome editing

Rights and Permissions

Copyright: © 2020 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

DOI of Published Version

10.17912/micropub.biology.000315

Source

Duan Y, Choi S, Nelson C, Ambros V. Engineering essential genes with a "jump board" strategy using CRISPR/Cas9. MicroPubl Biol. 2020 Oct 8;2020:10.17912/micropub.biology.000315. doi: 10.17912/micropub.biology.000315. PMID: 33274316; PMCID: PMC7704125. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

microPublication biology

PubMed ID

33274316

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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