University of Massachusetts Medical School Faculty Publications
Title
Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation [preprint]
UMMS Affiliation
RNA Therapeutics Institute; Department of Biochemistry and Molecular Pharmacology
Publication Date
2020-12-29
Document Type
Article Preprint
Disciplines
Molecular Biology | Nucleic Acids, Nucleotides, and Nucleosides | Structural Biology
Abstract
Frameshifting of mRNA during translation provides a strategy to expand the coding repertoire of cells and viruses. Where and how in the elongation cycle +1-frameshifting occurs remains poorly understood. We captured six ∼3.5-Å-resolution cryo-EM structures of ribosomal elongation complexes formed with the GTPase elongation factor G (EF-G). Three structures with a +1-frameshifting-prone mRNA reveal that frameshifting takes place during translocation of tRNA and mRNA. Prior to EF-G binding, the pre-translocation complex features an in-frame tRNA-mRNA pairing in the A site. In the partially translocated structure with EF-G, the tRNA shifts to the +1-frame codon near the P site, whereas the freed mRNA base bulges between the P and E sites and stacks on the 16S rRNA nucleotide G926. The ribosome remains frameshifted in the nearly post-translocation state. Our findings demonstrate that the ribosome and EF-G cooperate to induce +1 frameshifting during mRNA translocation.
Keywords
Molecular Biology, mRNA, ribosomes
Rights and Permissions
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
DOI of Published Version
10.1101/2020.12.29.424751
Source
bioRxiv 2020.12.29.424751; doi: https://doi.org/10.1101/2020.12.29.424751. Link to preprint on bioRxiv.
Journal/Book/Conference Title
bioRxiv
Repository Citation
Demo G, Loveland AB, Svidritskiy E, Gamper HB, Hou Y, Korostelev AA. (2020). Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation [preprint]. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1101/2020.12.29.424751. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1867
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Included in
Molecular Biology Commons, Nucleic Acids, Nucleotides, and Nucleosides Commons, Structural Biology Commons