UMass Chan Medical School Faculty Publications
Title
Genome information processing by the INO80 chromatin remodeler positions nucleosomes [preprint]
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology
Publication Date
2020-11-09
Document Type
Article Preprint
Disciplines
Amino Acids, Peptides, and Proteins | Biochemistry | Molecular Biology | Structural Biology
Abstract
The fundamental molecular determinants by which ATP-dependent chromatin remodelers organize nucleosomes across eukaryotic genomes remain largely elusive. Here, chromatin reconstitutions on physiological, whole-genome templates reveal how remodelers read and translate genomic information into nucleosome positions. Using the yeast genome and the multi-subunit INO80 remodeler as a paradigm, we identify DNA shape/mechanics encoded signature motifs as sufficient for nucleosome positioning and distinct from known DNA sequence preferences of histones. INO80 processes such information through an allosteric interplay between its core- and Arp8-modules that probes mechanical properties of nucleosomal and linker DNA. At promoters, INO80 integrates this readout of DNA shape/mechanics with a readout of co-evolved sequence motifs via interaction with general regulatory factors bound to these motifs. Our findings establish a molecular mechanism for robust and yet adjustable +1 nucleosome positioning and, more generally, remodelers as information processing hubs that enable active organization and allosteric regulation of the first level of chromatin.
Keywords
Biochemistry, genome information processing, chromatin
Rights and Permissions
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
DOI of Published Version
10.1101/2020.11.03.366690
Source
bioRxiv 2020.11.03.366690; doi: https://doi.org/10.1101/2020.11.03.366690. Link to preprint on bioRxiv.
Journal/Book/Conference Title
bioRxiv
Repository Citation
Oberbeckmann E, Krietenstein N, Niebauer V, Wang Y, Schall K, Moldt M, Straub T, Rohs R, Hopfner K, Korber P, Eustermann S. (2020). Genome information processing by the INO80 chromatin remodeler positions nucleosomes [preprint]. UMass Chan Medical School Faculty Publications. https://doi.org/10.1101/2020.11.03.366690. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1842
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Included in
Amino Acids, Peptides, and Proteins Commons, Biochemistry Commons, Molecular Biology Commons, Structural Biology Commons
Comments
This article is a preprint. Preprints are preliminary reports of work that have not been certified by peer review.
The PDF available for download is Version 2 of this preprint. The complete version history of this preprint is available at bioRxiv.