UMass Chan Medical School Faculty Publications
UMMS Affiliation
Program in Molecular Medicine
Publication Date
2013-05-15
Document Type
Article
Subjects
Dendritic Cells; Receptors, N-Methyl-D-Aspartate; Receptors, Neurotransmitter
Disciplines
Amino Acids, Peptides, and Proteins | Animal Experimentation and Research | Investigative Techniques | Nervous System | Neuroscience and Neurobiology | Nucleic Acids, Nucleotides, and Nucleosides
Abstract
Long-term synaptic plasticity involves changes in the expression and membrane insertion of cell-surface proteins. Interestingly, the mRNAs encoding many cell-surface proteins are localized to dendrites, but whether dendritic protein synthesis is required for activity-induced surface expression of specific proteins is unknown. Herein, we used microfluidic devices to demonstrate that dendritic protein synthesis is necessary for activity-induced insertion of GluN2A-containing NMDA receptors in rat hippocampal neurons. Furthermore, visualization of activity-induced local translation of GluN2A mRNA and membrane insertion of GluN2A protein in dendrites was directly observed and shown to depend on a 3' untranslated region cytoplasmic polyadenylation element and its associated translation complex. These findings uncover a novel mechanism for cytoplasmic polyadenylation element-mediated posttranscriptional regulation of GluN2A mRNA to control NMDA receptor surface expression during synaptic plasticity.
Rights and Permissions
Copyright 2013 the authors. Publisher PDF posted as allowed by the publisher's author rights policy at http://www.jneurosci.org/site/misc/ifa_policies.xhtml#copyright.
DOI of Published Version
10.1523/JNEUROSCI.0289-13.2013
Source
Swanger SA, He YA, Richter JD, Bassell GJ. Dendritic GluN2A synthesis mediates activity-induced NMDA receptor insertion. J Neurosci. 2013 May 15;33(20):8898-908. doi: 10.1523/JNEUROSCI.0289-13.2013. Link to article on publisher's site
Related Resources
Journal/Book/Conference Title
The Journal of neuroscience : the official journal of the Society for Neuroscience
PubMed ID
23678131
Repository Citation
Swanger SA, He YA, Richter JD, Bassell GJ. (2013). Dendritic GluN2A Synthesis Mediates Activity-Induced NMDA Receptor Insertion. UMass Chan Medical School Faculty Publications. https://doi.org/10.1523/JNEUROSCI.0289-13.2013. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/18
Included in
Amino Acids, Peptides, and Proteins Commons, Animal Experimentation and Research Commons, Investigative Techniques Commons, Nervous System Commons, Neuroscience and Neurobiology Commons, Nucleic Acids, Nucleotides, and Nucleosides Commons