Department of Biochemistry and Molecular Pharmacology
Amino Acids, Peptides, and Proteins | Genetics and Genomics | Molecular Biology
Despite the long-observed correlation between H3K9me3, chromatin architecture and transcriptional repression, how H3K9me3 regulates genome higher-order organization and transcriptional activity in living cells remains unclear. Here we develop EpiGo (Epigenetic perturbation induced Genome organization)-KRAB to introduce H3K9me3 at hundreds of loci spanning megabases on human chromosome 19 and simultaneously track genome organization. EpiGo-KRAB is sufficient to induce de novo heterochromatin-like domain formation, which requires SETDB1, a methyltransferase of H3K9me3. Unexpectedly, EpiGo-KRAB induced heterochromatin-like domain does not result in widespread gene repression except a small set of genes with concurrent loss of H3K4me3 and H3K27ac. Ectopic H3K9me3 appears to spread in inactive regions but is largely restricted to transcriptional initiation sites in active regions. Finally, Hi-C analysis showed that EpiGo-KRAB induced to reshape existing compartments. These results reveal the role of H3K9me3 in genome organization could be partially separated from its function in gene repression.
Molecular Biology, Genomics, H3K9me3
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DOI of Published Version
bioRxiv 2020.07.15.204719; doi: https://doi.org/10.1101/2020.07.15.204719. Link to preprint on bioRxiv service.
Feng Y, Wang Y, Wang X, He X, Yang C, Naseri A, Pederson T, Zheng J, Zhang S, Xiao X, Xie W, Ma H. (2020). Simultaneous Epigenetic Perturbation and Genome Imaging Reveal Distinct Roles of H3K9me3 in Chromatin Architecture and Transcription. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1101/2020.07.15.204719. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1741
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This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.