University of Massachusetts Medical School Faculty Publications
UMMS Affiliation
Program in Molecular Medicine; RNA Therapeutics Institute
Publication Date
2020-08-05
Document Type
Article Preprint
Disciplines
Cell and Developmental Biology | Enzymes and Coenzymes | Molecular Biology | Nucleic Acids, Nucleotides, and Nucleosides
Abstract
Eukaryotic cells regulate 5' triphosphorylated (ppp-) RNAs to promote cellular functions and prevent recognition by antiviral RNA sensors. For example, RNA capping enzymes possess triphosphatase domains that remove the γ phosphates of ppp-RNAs during RNA capping. Members of the closely related PIR1 family of RNA polyphosphatases remove both the β and γ phosphates from ppp-RNAs. Here we show that C. elegans PIR-1 dephosphorylates ppp-RNAs made by cellular RdRPs and is required for the maturation of 26G-RNAs, Dicer-dependent small RNAs that regulate thousands of genes during spermatogenesis and embryogenesis. PIR-1 also regulates the CSR-1 22G-RNA pathway and has critical functions in both somatic and germline development. Our findings suggest that PIR-1 modulates both Dicer-dependent and -independent Argonaute pathways, and provide insight into how cells and viruses use a conserved RNA phosphatase to regulate and respond to ppp-RNA species.
Keywords
molecular biology, RNA phosphatase PIR-1, endogenous small RNA pathways
Rights and Permissions
The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.
DOI of Published Version
10.1101/2020.08.03.235143
Source
bioRxiv 2020.08.03.235143; doi: https://doi.org/10.1101/2020.08.03.235143. Link to preprint on bioRxiv service.
Journal/Book/Conference Title
bioRxiv
Repository Citation
Chaves DA, Dai H, Li L, Moresco JJ, Eun Oh M, Conte D, Yates JR, Mello CC, Gu W. (2020). The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1101/2020.08.03.235143. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1736
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Included in
Cell and Developmental Biology Commons, Enzymes and Coenzymes Commons, Molecular Biology Commons, Nucleic Acids, Nucleotides, and Nucleosides Commons