Program in Molecular Medicine; RNA Therapeutics Institute
Cell and Developmental Biology | Enzymes and Coenzymes | Molecular Biology | Nucleic Acids, Nucleotides, and Nucleosides
Eukaryotic cells regulate 5' triphosphorylated (ppp-) RNAs to promote cellular functions and prevent recognition by antiviral RNA sensors. For example, RNA capping enzymes possess triphosphatase domains that remove the γ phosphates of ppp-RNAs during RNA capping. Members of the closely related PIR1 family of RNA polyphosphatases remove both the β and γ phosphates from ppp-RNAs. Here we show that C. elegans PIR-1 dephosphorylates ppp-RNAs made by cellular RdRPs and is required for the maturation of 26G-RNAs, Dicer-dependent small RNAs that regulate thousands of genes during spermatogenesis and embryogenesis. PIR-1 also regulates the CSR-1 22G-RNA pathway and has critical functions in both somatic and germline development. Our findings suggest that PIR-1 modulates both Dicer-dependent and -independent Argonaute pathways, and provide insight into how cells and viruses use a conserved RNA phosphatase to regulate and respond to ppp-RNA species.
molecular biology, RNA phosphatase PIR-1, endogenous small RNA pathways
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The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.
DOI of Published Version
bioRxiv 2020.08.03.235143; doi: https://doi.org/10.1101/2020.08.03.235143. Link to preprint on bioRxiv service.
Chaves DA, Dai H, Li L, Moresco JJ, Eun Oh M, Conte D, Yates JR, Mello CC, Gu W. (2020). The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1101/2020.08.03.235143. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1736
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This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.