UMass Chan Medical School Faculty Publications
UMMS Affiliation
Program in Bioinformatics and Integrative Biology; Program in Molecular Medicine
Publication Date
2020-04-18
Document Type
Article Preprint
Disciplines
Amino Acids, Peptides, and Proteins | Biochemistry, Biophysics, and Structural Biology | Biodiversity | Ecology and Evolutionary Biology | Enzymes and Coenzymes | Genomics | Immunology and Infectious Disease | Virology | Virus Diseases
Abstract
The novel coronavirus SARS-CoV-2 is the cause of Coronavirus Disease-2019 (COVID-19). The main receptor of SARS-CoV-2, angiotensin I converting enzyme 2 (ACE2), is now undergoing extensive scrutiny to understand the routes of transmission and sensitivity in different species. Here, we utilized a unique dataset of 410 vertebrates, including 252 mammals, to study cross-species conservation of ACE2 and its likelihood to function as a SARS-CoV-2 receptor. We designed a five-category ranking score based on the conservation properties of 25 amino acids important for the binding between receptor and virus, classifying all species from very high to very low. Only mammals fell into the medium to very high categories, and only catarrhine primates in the very high category, suggesting that they are at high risk for SARS-CoV-2 infection. We employed a protein structural analysis to qualitatively assess whether amino acid changes at variable residues would be likely to disrupt ACE2/SARS-CoV-2 binding, and found the number of predicted unfavorable changes significantly correlated with the binding score. Extending this analysis to human population data, we found only rare (
Keywords
ACE2, COVID-19, SARS-CoV-2, comparative genomics, evolution, host range, species conservation
Rights and Permissions
The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.
DOI of Published Version
10.1101/2020.04.16.045302
Source
Damas J, Hughes GM, Keough KC, Painter CA, Persky NS, Corbo M, Hiller M, Koepfli KP, Pfenning AR, Zhao H, Genereux DP, Swofford R, Pollard KS, Ryder OA, Nweeia MT, Lindblad-Toh K, Teeling EC, Karlsson EK, Lewin HA. Broad Host Range of SARS-CoV-2 Predicted by Comparative and Structural Analysis of ACE2 in Vertebrates. bioRxiv [Preprint]. 2020 Apr 18:2020.04.16.045302. doi: 10.1101/2020.04.16.045302. PMID: 32511356; PMCID: PMC7263403. Link to preprint on bioRxiv service
Related Resources
Journal/Book/Conference Title
bioRxiv
PubMed ID
32511356
Repository Citation
Damas J, Karlsson EK, Lewin HA. (2020). Broad Host Range of SARS-CoV-2 Predicted by Comparative and Structural Analysis of ACE2 in Vertebrates [preprint]. UMass Chan Medical School Faculty Publications. https://doi.org/10.1101/2020.04.16.045302. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1675
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Included in
Amino Acids, Peptides, and Proteins Commons, Biochemistry, Biophysics, and Structural Biology Commons, Biodiversity Commons, Ecology and Evolutionary Biology Commons, Enzymes and Coenzymes Commons, Genomics Commons, Immunology and Infectious Disease Commons, Virology Commons, Virus Diseases Commons
Comments
Now published in PNAS, doi:10.1073/pnas.2010146117, and available in eScholarship@UMMS.
Full author list omitted for brevity. For the full list of authors, see preprint.