Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute; Department of Radiology; Graduate School of Biomedical Sciences
Amino Acids, Peptides, and Proteins | Biochemistry, Biophysics, and Structural Biology | Microbiology | Nucleic Acids, Nucleotides, and Nucleosides
Argonaute proteins use nucleic acid guides to protect organisms against transposons and viruses. In the eubacterium Thermus thermophilus, the DNA-guided Argonaute TtAgo defends against transformation by DNA plasmids. Here, we report that TtAgo also participates in DNA replication. TtAgo binds small DNA guides derived from the chromosomal region where replication terminates and associates with proteins known to act in DNA replication. T. thermophilus deploys a single type II topoisomerase, gyrase. When gyrase is inhibited, T. thermophilus relies on TtAgo to complete replication of its circular genome; loss of both gyrase and TtAgo activity produces long filaments that fail to separate into individual bacteria. We propose that the primary role of TtAgo is to help T. thermophilus disentangle the catenated circular chromosomes made by DNA replication.
Microbiology, Argonaute Protein, DNA Replication, Thermus thermophilus
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The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.
DOI of Published Version
bioRxiv 869172; doi: https://doi.org/10.1101/869172. Link to preprint on bioRxiv service.
Jolly SM, I, , Zhang H, Strittmatter L, Hendricks GM, Dhabaria A, Ueberheide B, Zamore PD. (2019). A DNA-guided Argonaute Protein Functions in DNA Replication in Thermus thermophilus. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1101/869172. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1658
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