University of Massachusetts Medical School Faculty Publications

UMMS Affiliation

Department of Microbiology and Physiological Systems; Graduate School of Biomedical Sciences

Publication Date

2019-10-31

Document Type

Article Preprint

Disciplines

Amino Acids, Peptides, and Proteins | Bacteria | Bacterial Infections and Mycoses | Biochemical Phenomena, Metabolism, and Nutrition | Cellular and Molecular Physiology | Microbiology

Abstract

The mycobacterial cell wall is a dynamic structure that protects Mycobacterium tuberculosis and its relatives from environmental stresses. Modulation of cell wall metabolism under stress is thought to be responsible for decreased cell wall permeability and increased tolerance to antibiotics. The signaling pathways that control cell wall metabolism under stress, however, are poorly understood. Here, we examine the signaling capacity of a cell wall master regulator, the Serine Threonine Phosphatase PstP, in the model organism Mycobacterium smegmatis. We studied how interference with a regulatory phosphorylation site on PstP affects growth, cell wall metabolism and antibiotic tolerance. We find that a phospho-mimetic mutation, pstP T171E, slows growth, misregulates both mycolic acid and peptidoglycan metabolism in different conditions, and interferes with antibiotic tolerance. These data suggest that phosphorylation on PstP controls its substrate specificity and is important in the transition between growth and stasis.

Keywords

Microbiology, antibiotic tolerance, metabolism, Mycobacterium tuberculosis, Mycobacterium smegmatis, phosphorylation

Rights and Permissions

The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.

DOI of Published Version

10.1101/825588

Source

bioRxiv 825588; doi: https://doi.org/10.1101/825588. Link to preprint on bioRxiv service.

Journal/Book/Conference Title

bioRxiv

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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