Rapid phenotyping of cancer stem cells using multichannel nanosensor arrays
Department of Molecular, Cell and Cancer Biology; UMass Metabolic Network
Cancer Biology | Cell Biology | Nanomedicine | Neoplasms
Cancer stem cells (CSCs) contribute to multidrug resistance, tumor recurrence and metastasis, making them prime therapeutic targets. Their ability to differentiate and lose stem cell properties makes them challenging to study. Currently, there is no simple assay that can capture and trace the dynamic phenotypic changes on the CSC surface. Here, we report rapid discrimination of breast CSCs from non-CSCs using a nanoparticle-fluorescent-protein based sensor. This nanosensor was employed to discriminate CSCs from non-CSCs, as well CSCs that had differentiated in vitro in two breast cancer models. Importantly, the sensor platform could also discriminate CSCs from the bulk population of cells in patient-derived xenografts of human breast cancer. Taken together, the results obtained demonstrate the feasibility of using the nanosensor to phenotype CSCs and monitor their fate. Furthermore, this approach provides a novel area for therapeutic interventions against these challenging targets.
Array-based sensing, Cancer stem cell, Discrimination, Patient-derived xenografts, Personalized medicine
DOI of Published Version
Nanomedicine. 2018 May 17;14(6):1931-1939. doi: 10.1016/j.nano.2018.05.009. [Epub ahead of print]. Link to article on publisher's site
Nanomedicine : nanotechnology, biology, and medicine
Geng, Yingying; Goel, Hira Lal; Le, Ngoc B.; Yoshii, Tatsuyuki; Mout, Rubul; Tonga, Gulen Y.; Amante, John J.; Mercurio, Arthur M.; and Rotello, Vincent M., "Rapid phenotyping of cancer stem cells using multichannel nanosensor arrays" (2018). University of Massachusetts Medical School Faculty Publications. 1590.