University of Massachusetts Medical School Faculty Publications
Title
Rapid phenotyping of cancer stem cells using multichannel nanosensor arrays
UMMS Affiliation
Department of Molecular, Cell and Cancer Biology; UMass Metabolic Network
Publication Date
2018-05-17
Document Type
Article
Disciplines
Cancer Biology | Cell Biology | Nanomedicine | Neoplasms
Abstract
Cancer stem cells (CSCs) contribute to multidrug resistance, tumor recurrence and metastasis, making them prime therapeutic targets. Their ability to differentiate and lose stem cell properties makes them challenging to study. Currently, there is no simple assay that can capture and trace the dynamic phenotypic changes on the CSC surface. Here, we report rapid discrimination of breast CSCs from non-CSCs using a nanoparticle-fluorescent-protein based sensor. This nanosensor was employed to discriminate CSCs from non-CSCs, as well CSCs that had differentiated in vitro in two breast cancer models. Importantly, the sensor platform could also discriminate CSCs from the bulk population of cells in patient-derived xenografts of human breast cancer. Taken together, the results obtained demonstrate the feasibility of using the nanosensor to phenotype CSCs and monitor their fate. Furthermore, this approach provides a novel area for therapeutic interventions against these challenging targets.
Keywords
Array-based sensing, Cancer stem cell, Discrimination, Patient-derived xenografts, Personalized medicine
DOI of Published Version
10.1016/j.nano.2018.05.009
Source
Nanomedicine. 2018 May 17;14(6):1931-1939. doi: 10.1016/j.nano.2018.05.009. [Epub ahead of print]. Link to article on publisher's site
Related Resources
Journal/Book/Conference Title
Nanomedicine : nanotechnology, biology, and medicine
PubMed ID
29778888
Repository Citation
Geng, Yingying; Goel, Hira Lal; Le, Ngoc B.; Yoshii, Tatsuyuki; Mout, Rubul; Tonga, Gulen Y.; Amante, John J.; Mercurio, Arthur M.; and Rotello, Vincent M., "Rapid phenotyping of cancer stem cells using multichannel nanosensor arrays" (2018). University of Massachusetts Medical School Faculty Publications. 1590.
https://escholarship.umassmed.edu/faculty_pubs/1590